HPLC法测定双黄消炎片中盐酸小檗碱的含量  

Determination of berberine in shuanghuangxiaoyan tablets by HPLC

作　　者：周家才 梁玉梅 邱震 

机构地区：[1]南京医药合肥天星有限公司,安徽合肥230061

出　　处：《安徽医药》2009年第3期267-269,共3页Anhui Medical and Pharmaceutical Journal

摘　　要：目的采用高效液相色谱法测定双黄？肖炎片中盐酸小檗碱的含量。方法Kromasil C18色谱柱（250mm×4．6mm，5μm）；乙腈-0．05mol·L^-1磷酸二氢钾溶液（25：75）为流动相，流速为1．0ml·min^-1；检测波长为347am。结果盐酸小檗碱线性范围为9．2。146．4mg·L^-1（r=0．9999，n=5），平均回收率为99．93％，RSD=0．60％（n=6）。结论该方法准确、简便，可用于该制剂的质量控制。Aim To establish an HPLC method for determining berberine in shuanghuangxiaoyan tablets. Method C18 column（250 mm × 4.6 mm,5 μm） was used and the mobile phase was composed of acetonitrile-0.05 mol · L ^- 1KDP （ 25 ： 75 ）. The flow rate was 1.0 ml · min ^- 1. The detection wavelength was at 347 nm. Result The linear range of berberine was from 9.2 to 146.4 mg· L ^- 1 （ r = 0. 999 9, n = 5 ） and the average recovery was 99.93 % with an RSD of 0.60% （ n = 6）. Conclusion The method is accurate and simple for quality control of the preparation.

关 键 词：高效液相色谱法 盐酸小檗碱 双黄消炎片 

分 类 号：R286.0[医药卫生—中药学] R725.631[医药卫生—中医学]