不锈钢微滤膜在5′-核苷酸生产过程中的应用  

Application of Stainless Steel Microfiltration Membrane in Production of 5′-Nucleotides

作　　者：陈琦[1] 吕浩[1] 张业勇[1] 

机构地区：[1]南京工业大学制药与生命科学学院材料化学工程国家重点实验室,江苏南京210009

出　　处：《化学与生物工程》2009年第3期53-57,共5页Chemistry & Bioengineering

基　　金：国家973计划资助项目(2007CB714305);国家863计划资助项目(2007AA100404)

摘　　要：采用不锈钢微滤膜,对酶解RNA制备5′-核苷酸中杂蛋白的分离工艺进行了研究。考察了压力、温度对膜渗透通量、杂蛋白截留率以及透过液中杂蛋白含量等的影响,确定最佳工艺操作条件为:压力3.8 kg.cm-2、温度20℃,此时杂蛋白截留率达到66.7%、膜透过液中杂蛋白含量约为0.1 mg.mL-1。研究表明,滤饼层模型能较好地解释不锈钢膜的膜污染问题,通过考察膜渗透通量的恢复率,确定了最佳膜清洗方案,整个清洗过程只需要110 min,操作简捷,膜渗透通量能完全恢复。Stainless steel microfiltration membrane was applied to remove the protein in the enzymatic hydrolysate of 5′-nucleotides production. The effects of pressure and temperature on permeate flux, protein rejection rate and protein level in permeate were studied. The optimum condition of pressure at 3.8 kg · cm^-2 and temperature of 20℃ was obtained. The protein rejection rate reached to 66.7% as well as the protein level in permeate was about 0.1 mg · mL^-1. Cake filtration model was in good agreement with the experimental data. By investigating of the effects of a series of cleaning agents and cleaning time on the recovery of permeate flux, the best solution was evaluated. The whole cleaning task only lasted 110 rain and the permeate flux could be regenerated completely.

关 键 词：微滤膜 5′-核苷酸 污染 再生 

分 类 号：TQ028.8[化学工程]