光谱法研究卟啉锌(HP—Zn)配合物在超声波作用下对牛血清白蛋白(BSA)的损伤  

Study on damage of BSA in the presence of HP—Zn complex under ultrasonic irradiation by spectrophtomerty

作　　者：刘彬[1] 张媛媛[2] 王君[1,2] 王新[1] 刘丽君[2] 郭颖[1] 张利群[1] 

机构地区：[1]辽宁大学药学院,辽宁沈阳110036 [2]辽宁大学化学院,辽宁沈阳110036

出　　处：《渤海大学学报（自然科学版）》2009年第1期5-12,共8页Journal of Bohai University:Natural Science Edition

基　　金：国家自然科学基金资助项目(No:20371023);辽宁省教育厅自然科学基金资助项目(No:2008S101;No.2008247)

摘　　要：应用荧光光谱和紫外-可见光谱研究了卟啉锌（HP—Zn）与牛血清白蛋白（BSA）的相互作用及在超声波作用下HP—Zn对BSA的损伤作用，并探讨了超声照射时间，HP—Zn浓度和溶液酸度等因素对BSA损伤的影响。结果表明，37℃时，BSA与HP—Zn的结合数n=1．0379，其平衡常数KA=6．2661×10^4，两者之间的作用距离r=3．53nm。在一定条件下，BSA的损伤程度随着超声照射时间．HP—Zn浓度和溶液酸度的增大而增大。这一结果对声动力学疗法（SDT）治疗肿瘤应用于临床具有重要的意义。The interaction between hematopotphyrin zinc （HP-Zn） and bovine serum albumin （BSA） and damage of BSA in the presence of HP-Zn under ultrasonic irradiation were studied by UV -Vis and fluorescence spectra. The influences of ultrasonic irradiation time, HP-Zn concentration and solution acidity on damage of BSA were also detected. The results showed that at 37℃, the binding site number （n）, the apparent binding constant （KA） and the binding distance （r） were 1. 0379, 6. 2661 × 10^4and 3.53 nm, respectively. Under different conditions, the damage degree of BSA rose up with the increasing of ultrasonic irradiation time, HP-Zn concentration and solution pH value. The research results are of great significance for driving sonodynamic treatment （SDT） to clinic application.

关 键 词：超声波 卟啉锌(HP—Zn) 牛血清白蛋白(BSA) 相互作用 损伤 

分 类 号：O614.241[理学—无机化学] O657.3[理学—化学]