低剂量铅对大鼠脑组织一氧化氮合酶表达及活性的影响  

Effect of Low Level Lead on Expression and Activity of Nitric Oxide Synthase in Rat Brain

作　　者：宋丽华[1] 张亨山[1] 赵西龙[1] 郭润荣[1] 秦钰慧[1] 

机构地区：[1]中国预防医学科学院环境卫生监测所,北京100021

出　　处：《广东微量元素科学》1998年第4期21-24,共4页Trace Elements Science

摘　　要：为探讨低水平铅损伤学习记忆功能的作用机理，采用NADPH—黄递酶组织化学方法和3H—瓜氨酸生成生物检测技术，观察了新生大鼠在400mg／L醋酸铅染毒40天后对脑组织内一氧化氮合酶表达及活性的影响。结果显示大脑皮质一氧化氮合酶阳性神经无数量明显减少（P＜0．05），在海马区脑组织内一氧化氮合酶活性降低54．4％。结论：低水平铅暴露后可抑制大鼠脑组织内一氧化氮合酶的表达及活性。To inveshgate the mechanism of effect of lead on learning and memory, the activity and distribution of nitric oxide synthase in neonatal rat brain exposed to 400mg/L lead acetate for 40 days were observed by means of NADPH-diaphorase histochendstry and 3H-citrulline generation bioassay. The results showed that the number of nitric oxide synthase positive neumns in the cerebral cortex was significantly reduced (P < 0.05), and nitric oxide synthase achvity in the hippocampus was decreased by 54.4% compared to the control group. Conclusion: the expression and activity of nitric oxide synthase were suppressed remarkably in brain of rats after exposure to low level of lead.

关 键 词：铅 一氧化氮合酶 脑组织 活性 脑损伤 

分 类 号：R595.2[医药卫生—内科学] R742[医药卫生—临床医学]