反高效液相色谱法研究芹菜素在大鼠体内的药代动力学及组织分布  

Study on pharmacokinetics and tissue distribution of apigenin in rats by RP-HPLC

作　　者：张宇[1] 常佩亮[1] 夏薇[1] 吴坤[1] 赵秀娟[1] 

机构地区：[1]哈尔滨医科大学公共卫生学院营养与食品卫生教研室,黑龙江哈尔滨150081

出　　处：《中华疾病控制杂志》2009年第1期79-82,共4页Chinese Journal of Disease Control & Prevention

基　　金：黑龙江省卫生厅资助项目(2004-225)

摘　　要：目的建立一个检测大鼠血浆及组织中芹菜素的RP-HPLC分析方法,并对其大鼠体内过程特性进行分析研究。方法生物样品经甲醇沉淀蛋白质,采用RP-HPLC法,色谱柱为Phe-nomenexC18柱(250×4.6mm,4μm),流动相为甲醇-0.1%磷酸水溶液-乙腈(25∶40∶35),流速1.0ml/min,柱温为30℃,检测波长为339nm。结果测得芹菜素在血浆和组织中的线性范围分别为:0.10～2.00μg/ml(r>0.999)和0.05～2.00μg/g(r>0.999),最低检出限分别为0.03μg/ml和0.02μg/g,日内和日间精密度(RSD)均小于8%。大鼠一次性灌胃给予芹菜素后血芹菜素浓度-时间曲线呈一室模型,半衰期t1/2(116.53±22.55)min。在主要组织脏器分布特点是C肝>C肾>C肺>C卵>C脂肪>C脾>C脑>C心>C睾。结论本法准确、灵敏度较高,可用于芹菜素在大鼠体内代谢过程的研究。Objective To establish an RP-HPLC method for the analysis and detection of apigenin in plasma and tissue of rats. Methods The biological samples were diluted, by which also precipitated the protein in plasma. The Phenomenex Ct8 column（250 × 4.6 mm, 4 μm） was used as analytical column. The mobile phase consisted of methanol-0. 1% acetic acid-acetonitrite （25 ： 40 ： 35） at a flow rate of 1.0 ml/min. The column temperature was 30 ℃ . The UV detection wavelength was 339 nm. Results The linear range of apigenin in plasma and tissue were 0.10-2.00 μg/ml （r〉0. 999） and 0.05 -2.00 μg/g （r〉0.999）. The lowest detectable concentration of apigenin were 0.03 μg/ml and 0.02 μg/g. RSD of intra-day and inter-day were all less than 8 % . After a single oral dose of apigenin, the concentration of apigenin appeared as one-compartment model and in order Clive 〉 Ckidney 〉 Clung 〉 Covary〉Cfat〉Cspleen〉Cbrain〉Cheart 〉Ctesticle. Conclusions The method is accurate and reliable. It can be used for the pharmacokinetic studies of apigenin in rats.

关 键 词：芹菜素 药代动力学 组织分布 色谱法 高压液相 

分 类 号：R332[医药卫生—人体生理学] R284.1[医药卫生—基础医学]