白藜芦醇体外抗血小板作用及机制初探  

Primary Study of Suppressive Effect and Mechanism of Resveratrol on Platelet Aggregation

作　　者：杨雨民 王世君[2] 王宏强[3] 王兴祥[2] 陈君柱[2] 

机构地区：[1]内蒙古中蒙医院心内科,呼和浩特010020 [2]浙江大学附属第一医院心内科,杭州310003 [3]浙江大学医学院病理和病理生理学系,杭州310031

出　　处：《中国药学杂志》2009年第3期191-194,共4页Chinese Pharmaceutical Journal

基　　金：浙江省中医药重点研究计划资助项目(2007ZA015)

摘　　要：目的探讨白黎芦醇(Resveratrol,Resv)对腺苷二磷酸(adenosine diphosphate,ADP)诱导健康志愿者血小板聚集的抑制作用及可能机制。方法应用血小板聚集仪、流式细胞仪及磷屏成像仪,研究Resv和蛋白激酶C抑制剂(protein kinase C inhibitor,PKCI)对ADP诱导的健康志愿者血小板最大聚集率(maximal platelet aggregation rate,MPAG)、血小板膜结合纤维蛋白原(platelet membrane-bound fibrinogen,PFIG)、血小板胞膜蛋白激酶C活性(protein kinase C activity of membrane in platelet,M-PKC)及M-PKC占总PKC活性(total PKC activity in platelet,T-PKC)百分比(M-PKC/T-PKC)的影响。结果Resv(终浓度为25、50和100μmol·L-1)剂量依赖性地抑制ADP诱导的MPAG及PFIG;PKCI与Resv对MPAG及PFIG的抑制有相加作用;Resv(50μmol·L-1)可明显降低M-PKC活性及M-PKC/T-PKC活性的百分比。结论Resv可抑制ADP诱导的MPAG及PFIG,有明确的抗血小板作用;其机制可能与抑制血小板内PKC活性有关。OBJECTIVE To observe the effects and mechanism of Resveratrol （Resv） on adenosine diphosphate （ADP）-induced platelet aggregation. METHODS Platelet aggregometer, flow cytometry and phosphorimaging system were used to study the effects of Resv and protein kinase C inhibitor （PKCI） on the ADP-induced maximal platelet aggregation rate （MPAG）, platelet membrane- bound fibrinogen （PFIG）, the activity of protein kinase C in membrane of platelets （M-PKC）, and the rate of M-PKC in total PKC activity （T-PKC）in healthy volunteers. RESULTS Resv （25, 50 and 100μmol. L^-1） inhibited ADP-induced MPAG and PFIG in a dose-dependent manner. PKCI and Resv had additive effect in inhibiting MPAG and PFIG. Resv （50 μmol.L^-1） obviously depressed M-PKC and M-PKC/T-PKC. CONCLUSION Resv inhibits MPAG and PFIG, which mechanisms may be partly relative to the decrease of the activity of PKC of platelets.

关 键 词：白藜芦醇 血小板聚集 流式细胞术 蛋白激酶C 磷屏成像仪 

分 类 号：R965[医药卫生—药理学]