RP-HPLC同时测定紫金龙中4种异喹啉生物碱的含量  

Simultaneous Determination of Four Isoquinoline Alkaloids in Root of Dactylicapnos scandens by RP-HPLC

作　　者：张文鑫[1,2] 韩丽妹[1] 周毅生[2] 王建新[1] 

机构地区：[1]复旦大学药学院,上海200032 [2]河南中医学院药学院,郑州450008

出　　处：《中国药学杂志》2009年第3期233-236,共4页Chinese Pharmaceutical Journal

基　　金：上海市中西部科技合作基金(64358028)

摘　　要：目的建立同时测定紫金龙药材4种异喹啉类生物碱含量的RP-HPLC方法。方法采用Hypersil BDS C18色谱柱(4.6mm×250mm,5μm),流动相为梯度洗脱,A相:0.2%磷酸水溶液(三乙胺调pH为7.0),B相:甲醇;流速:1mL·min-1;检测波长:265nm;柱温:30℃。结果4种生物碱可达到基线分离。青藤碱、原阿片碱、紫堇定和异紫堇定的线性范围分别为0.0073～0.0657μg,0.1722～4.304μg,0.486～4.86μg和0.4870～9.7392μg,相关系数r均大于0.9990。平均加样回收率分别为97.84%(RSD=0.89%)、98.98%(RSD=0.58%)、97.14%(RSD=1.13%)和97.65%(RSD=0.74%)。结论本法精确、简便、重现性好,可用于对紫金龙药材及其制剂的质量控制。OBJECTIVE To establish a RP-HPLC method for the simultaneous determination of four isoquinoline alkaloids in the root ofDactylicapnos scandens. METHODS The samples was separated on a Hypersil ODS C18 column （4.6 mm ×250 mm, 5 μm） with gradient elution. The mobile phase was composed of methanol and 0.2% phosphoric acid（adjust to pH 7.0 with triethylamine）. The flow rate was 1.0 mL.min^-1 and the UV absorbance detection was set at 265 nm. RESULTS Four alkaloids were separated well. Good linearies were obtained within the range of 0.007 3-0.065 7, 0.172 2-4.304, 0.486-4.86 μg and 0.4870 -9.739 2 μg for sinomenine, protopine, corydine and isocorydine respectively. The average recoveries were 97.84% （RSD=0.89%）, 98.98%（RSD=0.58%）, 97.14% （RSD=1.13%） and 97.65%（RSD=0.74%）. CONCLUSION The method is accurate, reliable and convenient. It can be used to control the quality ofDactylicapnos scandens and its products.

关 键 词：高效液相色谱法 紫金龙 青藤碱 原阿片碱 紫堇定 异紫堇定 含量测定 

分 类 号：R917[医药卫生—药物分析学]