偃麦草属E染色体组特异SCAR标记对Lr19的特异性和稳定性研究  

Specificity and stability of E-chromosome specific SCAR marker from Thinopyrum spp. for Lr19

作　　者：闫红飞[1] 杨文香[1] 陈云芳[1] 孟庆芳[1] 刘大群[1] 

机构地区：[1]河北农业大学植物病理学系河北省农作物病虫害生物防治工程技术研究中心,保定071001

出　　处：《植物病理学报》2009年第1期76-81,共6页Acta Phytopathologica Sinica

基　　金：河北省自然科学基金资助项目(c2006000438);河北省农作物病虫害生物防治中心资助

摘　　要：小麦抗叶锈基因Lr19来源于长穗偃麦草,表现优良的抗叶锈性,国内外发现对Lr19有毒性的小麦叶锈菌菌株的报道较少。本研究以TcLr19和Thatcher亲本以及TcLr19×ThatcherF2代单株构建的分离群体为材料,建立了与Lr19共分离的稳定的SCAR分子标记,命名为Y19SCAR982。对49个小麦抗叶锈近等基因系材料的稳定性检测结果表明,其重复性好且为Lr19特异的分子标记。对120个小麦品种检测的结果表明,该标记可有效应用于小麦抗叶锈分子辅助选择育种。Leaf rust resistance gene Lr19 transferred from Thinopyrum elongatum into wheat cultivars has expressed resistance to most pathotypes of Puccinia triticina in China. To identify the molecular marker linked to Lr19, a segregating F2 population from a cross between the resistance parent TcLr19 and susceptible parent Thatcher were screened. A SCAR marker cosegregating with Lrl9 was found and named as Y19SCAR982. The result of stability validation test on the 49 international set of near-isogenic lines in Thatcher background suggested that Y19SCAR982 was specific and sufficient for Lr19. The detection of the marker in 120 wheat varieties indicated that it could be effectively used for MAS in breeding of the resistance to leaf rust in wheat.

关 键 词：长穗偃麦草 抗叶锈基因 LR19 SCAR标记 

分 类 号：S432.44[农业科学—植物病理学]