进境豇豆种子携带种传病毒的检测与鉴定  

Detection and identification of seed transmitted virus from imported cowpea germplasm

作　　者：陈青[1] 林石明[1] 陈红运[1] 廖富荣[1] 余芳平[1] 陈洪俊[2] 朱水芳[2] 

机构地区：[1]厦门出入境检验检疫局,厦门361012 [2]中国检验检疫科学研究院,北京100029

出　　处：《植物病理学报》2009年第1期91-94,共4页Acta Phytopathologica Sinica

基　　金：公益性行业科研专项(200810790);国家质检总局项目(2007IK253)

摘　　要：Imported cowpea seeds were detected with growing test,ELISA assay and RT-PCR method.The ELISA results showed that cowpea seedlings with symptoms reacted positively with antibody against Southern bean mosaic virus(SBMV).The 979 bp of fragment could be amplified from two positive ELISA samples using primers specific for Southern cowpea mosaic virus(SCPMV),and the sequence determination results proved that the pathogen existing in imported cowpea seeds was SCPMV.The positive ELISA results with SBMV antibody could be further confirmed by RT-PCR amplification with specific primers designed to amplify the coat protein gene and 3’ noncoding region of SCPMV and SBMV.The RT-PCR method presented here was suitable for molecular identification of SBMV and SCPMV in entry-exit plant quarantine laboratories.Imported cowpea seeds were detected with growing test, ELISA assay and RT-PCR method. The ELISA results showed that cowpea seedlings with symptoms reacted positively with antibody against Southern bean mosaic virus （SBMV）. The 979 bp of fragment could be amplified from two positive ELISA samples using primers specific for Southern cowpea mosaic virus （SCPMV）, and the sequence determination results proved that the pathogen existing in imported cowpea seeds was SCPMV. The positive ELISA results with SB- MV antibody could be further confirmed by RT-PCR amplification with specific primers designed to amplify the coat protein gene and 3＇ noncoding region of SCPMV and SBMV. The RT-PCR method presented here was suitable for molecular identification of SBMV and SCPMV in entry-exit plant quarantine laboratories.

关 键 词：种传病毒 豇豆种子 鉴定 检测 进境 病毒病害 植物病毒 初侵染来源 

分 类 号：S432.41[农业科学—植物病理学] S643.4[农业科学—农业昆虫与害虫防治]