香蕉线条病毒ORFⅡ基因的原核表达及抗体制备  

Prokaryotic expression and preparation of antiserum of Banana streak virus ORFⅡ gene

作　　者：何云蔚[1] 陈秀[1] 阮小蕾[1] 刘福秀[1] 李华平[1] 

机构地区：[1]华南农业大学植物病毒研究室,广州510642

出　　处：《植物病理学报》2009年第1期100-103,共4页Acta Phytopathologica Sinica

基　　金：国家自然科学基金资助项目(30671358);广东省自然科学基金资助项目(C036845)

摘　　要：ORFⅡ gene of Banana streak virus GuangDong isolate(BSV-GD) was amplified from a BSV-GD recombinant plasmid by PCR,and the gene was expressed by being cloned into prokaryote expression vector pET-28b(+).The fusion protein was about 16.5 kDa in size and was soluble with SDS-PAGE analysis.The purified protein was obtained by using the histidine labeling kit of N-terminus of protein.The antiserum was obtained by immunizing healthy rabbits with the purified protein.Western blot and ELISA analysis showed that the special antiserum of BSV possessed high titer,which was tested as 1∶51 200.The study was a base for further research on BSV including ORFⅡ gene function and virus detection.ORFⅡ gene of Banana streak virus GuangDong isolate （BSV-GD） was amplified from a BSVGD recombinant plasmid by PCR, and the gene was expressed by being cloned into prokaryote expression vector pET-28b（ ＋ ）. The fusion protein was about 16.5 kDa in size and was soluble with SDS-PAGE analysis. The purified protein was obtained by using the histidine labeling kit of N-terminus of protein. The antiserum was obtained by immunizing healthy rabbits with the purified protein. Western blot and ELISA analysis showed that the special antiserum of BSV possessed high titer, which was tested as 1：51 200. The study was a base for further research on BSV including ORF Ⅱ gene function and virus detection.

关 键 词：香蕉生产 病毒病 抗体制备 原核表达 线条 Ⅱ基因 ORF 植株矮化 

分 类 号：S668.1[农业科学—果树学] S436.412.1[农业科学—园艺学]