蛋白酶激活受体2介导肥大细胞IL-4分泌  

Proteinase activated receptor-2 promotes IL-4 release from mast cells

作　　者：张慧云[1] 林青[2] 林丽艳[2] 何韶衡[2,3] 

机构地区：[1]海南医学院病理生理学教研室,海口571101 [2]汕头大学医学院病理生理学教研室,汕头515041 [3]南京医科大学第一附属医院临床实验研究中心,南京210029

出　　处：《中国免疫学杂志》2009年第3期213-215,220,共4页Chinese Journal of Immunology

基　　金：国家自然科学基金资助项目(30471601;30570813)

摘　　要：目的:探讨蛋白酶激活受体2(Proteinase activated receptor-2,PAR-2)的激活对肥大细胞P815介质分泌的影响。方法:肥大细胞培养后用PAR-2激动肽,胰蛋白酶、类胰蛋白酶、弹性蛋白酶结合PAR-2拮抗肽激发肥大细胞,收集上清并用ELISA方法检测组胺、IL-4和IL-6的水平。结果:PAR-2激动肽、胰蛋白酶、类胰蛋白酶以浓度依赖的方式促进肥大细胞P815分泌IL-4。PAR-2拮抗肽能阻断胰蛋白酶和类胰蛋白酶引起的肥大细胞IL-4分泌(P<0.05)。胰蛋白酶、类胰蛋白酶以及PAR-2激动肽对肥大细胞IL-6的分泌和组胺释放无明显影响。弹性蛋白酶对肥大细胞IL-4、IL-6分泌及组胺释放功能无影响。结论:PAR-2的激活介导肥大细胞P815分泌IL-4;胰蛋白酶和类胰蛋白酶刺激肥大细胞IL-4分泌的发现为肥大细胞激活的自我放大机制提供了新的理论依据。Objective：To investigate the effect of activation of proteinase activated receptor（PAR）2 on mediator release from mast cells.Methods：P815 cells（a mast cell line） were challenged with various concentrations of PAR-2 agonist peptide,trypsin,tryptase or elasetase with or without PAR-2 antagonist peptide.The supernatants were collected and analyzed by enzyme-linked immunosorbent assay（ELISA） to detect the quantity of released IL-4,IL-6 and histamine.Results：PAR-2 agonist peptide,trypsin or tryptase induced a concentration-dependent IL-4,but not histamine release from P815 mast cells.Trypsin and tryptase induced IL-4 release from the mast cells was blocked by addition of PAR-2 antagonist peptide.No IL-4,IL-6 and histamine release was observed when P815 cells were incubated with elasetase.Conclusion：Induction of IL-4 release from mast cells by trypsin and tryptase through activation of PAR-2 added some novel information on the hypothesis of self-amplification mechanism of mast cell activation.

关 键 词：肥大细胞 PAR-2 胰蛋白酶 类胰蛋白酶 IL-4 

分 类 号：R392.8[医药卫生—免疫学]