4T1荷瘤小鼠肿瘤相关巨噬细胞(TAM)的表型及吞噬功能的研究  

Phenotypic analysis and phagocytic capacity of tumor-associated macrophages in 4T1 tumor-bearing mice

作　　者：郭强[1] 李康[1] 徐林[1] 蒋正刚[1] 徐薇[1] 储以微[1] 熊思东[1] 

机构地区：[1]复旦大学免疫生物学研究所上海医学院免疫学系,上海200032

出　　处：《中国免疫学杂志》2009年第3期225-228,233,共5页Chinese Journal of Immunology

基　　金：上海市医学领军人才基金(LJ06011);国家自然科学基金(30671952;30571713)资助

摘　　要：目的:研究小鼠乳腺癌实验动物模型中,荷瘤晚期肿瘤相关巨噬细胞的表型和功能,并探讨其与M2型巨噬细胞的关系。方法:从4T1荷瘤4周的雌性BALB/c小鼠中获取肿瘤相关巨噬细胞,用RT-PCR方法检测其巨噬细胞相关分子CCL3、CCL22、iNOS和Arg I的表达水平,用FACS检测巨噬细胞中CD16/32+和CD206+细胞所占比例,并通过酵母菌吞噬试验评估其功能。结果:肿瘤相关巨噬细胞与荷瘤小鼠的脾脏巨噬细胞相比,表达较高水平的CCL22和CD206,并且对酵母菌的吞噬能力显著下降。结论:4T1荷瘤4周小鼠肿瘤相关巨噬细胞在表型和功能上倾向于替代性活化的巨噬细胞。Objective：To study the phenotypes and functions of tumor-associated macrophages in 4T1 tumor-bearing mice.Methods：Tumor-associated macrophages and splenic macrophages were isolated from 4T1-bearing（4 weeks） BALB/c mice.Expression levels of CCL3,CCL22,iNOS and Arg I in the macrophages were detected by RT-PCR.The percentage of CD16/32＋ and CD206＋ cells was determined by FACS.Phagocytic capacity was assessed by yeast phagocytosis assay.Results：The expression of CCL22 and CD206 in tumor-associated macrophages was dramatically upregulated in contrast with that in splenic macrophages of 4T1-bearing mice,and the phagocytic capacity of tumor-associated macrophages was dramatically decreased.Conclusion：Tumor-associated macrophages in 4T1 tumor-bearing BALB/c mice might be polarized to the alternative activation phenotype（M2）.

关 键 词：肿瘤相关巨噬细胞 替代性活化巨噬细胞 吞噬功能 

分 类 号：R392.12[医药卫生—免疫学]