IL-23与IL-17在强直性脊柱炎患者中表达的初步研究  

The study on expression of IL-23 and IL-17 in ankylosing spondylitis

作　　者：王新卫[1] 林智明[1] 廖泽涛[1] 魏秋静[1] 江颖娟[1] 古洁若[1] 

机构地区：[1]中山大学附属第三医院风湿科,广州510630

出　　处：《中国免疫学杂志》2009年第3期266-270,共5页Chinese Journal of Immunology

基　　金：国家自然科学基金资助项目(No.30325019)

摘　　要：目的:通过研究IL-23与IL-17在强直性脊柱炎(AS)患者中的表达情况,为进一步阐明AS发病机制和寻找新的治疗靶点提供理论依据。方法:AS患者与健康对照血清及培养的外周血单个核细胞(PBMCs)上清中IL-23与IL-17水平应用ELISA方法检测;应用RT-PCR方法检测PBMCs中IL-23p19 mRNA的表达。结果:39例活动期AS患者血清IL-23与IL-17水平分别为(1 159.71±139.45)pg/ml和(172.21±73.81)pg/ml,均较健康对照明显升高(P<0.001);AS患者培养的PBMCs上清IL-23与IL-17水平分别为(108.63±34.53)pg/ml和(134.59±38.32)pg/ml,明显高于健康对照(P<0.001),IL-23p19 mRNA表达明显高于健康对照,平均光密度分析差异有极显著统计学意义(P<0.001);IL-23可促进健康对照和AS患者的PBMCs IL-17的分泌,此作用在AS患者更显著。结论:IL-23与IL-17可能在AS的发病中发挥作用,IL-23可能通过诱导IL-17的产生而使后者在AS的发病中发挥作用。Objective：To study the expression of IL-23 and IL-17 in ankylosiong spondylitis（AS） patients and to offer theoretical basis for clarifying the pathogenesis and searching new therapy target of AS.Methods：IL-23 and IL-17 amount in serum and supernatants of cultured peripheral blood mononuclear cells（PBMCs） from AS patients and healthy volunteers were measured by ELISA.IL-23p19 mRNA expression in PBMCs was detected by RT-PCR.Results：Serum IL-23 and IL-17 of 39 AS patients at active stage were（1 159.71±139.45） pg/ml and（172.21±73.81） pg/ml respectively;significantly higher than that of healthy control（P〈0.001）.IL-23 and IL-17 levels in supernatants of cultured PBMCs of AS patients were（108.63±34.53） pg/ml and（134.59±38.32） pg/ml respectively;significantly higher than that in healthy control subjects（P〈0.001）.The IL-23p19 mRNA expression in PBMCs were significantly higher in AS patients than in healthy control.Exogenous IL-23 evidently promoted production of IL-17 of the PBMCs from both normal individuals and AS patients.Conclusion：IL-23 and IL-17 may be involved in pathogenesis of AS.IL-23 may play a critical role in the development of AS through inducing production of IL-17.

关 键 词：白细胞介素23 白细胞介素17 强直性脊柱炎 

分 类 号：R593.23[医药卫生—内科学]