粘附分子CD44在支气管哮喘发病中的实验研究  

Investigation on the effects of CD44 in lung tissue in allergy-induced rat asthmatic model

作　　者：李羚[1] 杨莉[2] 唐珩[2] 金蕊[2] 

机构地区：[1]南京医科大学附属无锡市儿童医院呼吸科,无锡214023 [2]东南大学附属临床医学院儿科,南宁210009

出　　处：《中国免疫学杂志》2009年第1期80-83,86,共5页Chinese Journal of Immunology

基　　金：东南大学科学基金项目(No.9247341175)

摘　　要：目的:观察不同时段哮喘大鼠肺组织粘附分子CD44在转录与蛋白水平的表达,探讨其在哮喘发病中的作用机制。方法:以卵蛋白致敏制备大鼠哮喘模型,分别在激发后的一周、两周取肺组织,采用逆转录聚合酶链反应(Reverse tran-scription-polymerase chain reaction,RT-PCR)和免疫组织化学S-P方法研究肺组织CD44的表达;收集肺泡灌洗液(Bronchoalveolar lavage fluid,BALF)行细胞分类计数,肺组织石蜡切片行HE染色。结果:正常大鼠肺组织CD44有少量表达,在激发哮喘后一周肺组织CD44的表达显著升高(P<0.05),两周后升高更加显著(P<0.001)。哮喘大鼠BALF中炎性细胞明显增多(P<0.05),CD44在mRNA和蛋白水平与BLAF中淋巴细胞和嗜酸性粒细胞的百分比呈显著正相关。结论:哮喘肺组织CD44的过度表达与哮喘的发生发展密切相关,并在早期即开始参与哮喘的发病。CD44与嗜酸性粒细胞、淋巴细胞向哮喘气道炎症组织趋化浸润密切相关。Objective：To observe the expression of cell adhesion molecule CD44 in lung tissue of rats with asthma.Methods：32 SD rats were randomly divided into 4 groups：the normal group and the model group,8 rats in each group.The asthmatic model was established by repeated inhalation of ovalbulium.The expression of CD44 in lung tissue of rats was detected by semi-quantitatively reverse transcription-polymerase chain reaction（RT-PCR） and by means of immuno-histochemical staining.Differential leukocytes counts（neutrophils,eosinophils,and lymphocytes） in bronchoalveolar lavage fluid（BALF） were performed after staining with wright staining.Results：The expressions of CD44 increased one week after OVA challenge（P〈0.05） and much more significantly after OVA challenge two weeks（P〈0.01） than in the normal group.There was a significant increase of lymphocytes and eosinophils in BALF in model group was compared with those in normal group（P〈0.05）.CD44 expression was correlated significantly with the degree of leukocytes in BALF in model group.Conclusion：In the rat with asthma,cell adhesion molecule CD44 has an important role in the cascade of inflammation and the development.CD44 has an important function on promoting leukocyte recruitment to the asthmatic lungs.

关 键 词：哮喘 CD44 粘附分子 

分 类 号：R725.6[医药卫生—儿科]