库仑电化学法测定葛根中葛根素的方法学研究  

Methodological Study of Puerarin Content in Radix Puerariae by Coulomb Electrochemical Determination

作　　者：王振华[1] 孙琳[1] 魏智强[1] 田军[1] 孔秋玲[1] 潘艳[1] 

机构地区：[1]广州中医药大学,广州510006

出　　处：《中药新药与临床药理》2009年第2期140-142,共3页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：广东省科技攻关项目(2005B33001037)

摘　　要：目的建立葛根素的电化学含量测定方法。方法采用库仑阵列检测器研究葛根素的电化学行为及含量测定方法。结果在pH4.32磷酸盐缓冲液中,葛根素在0.16～162ng内与峰面积呈良好线性关系,回归方程为A=1444.7C+88.635,相关系数r=0.9998,平均回收率为96.88%,RSD为1.75%。结论电化学法操作简便,准确,灵敏度高,可用于葛根素的含量测定以及生物样品的测定。Objective To set up the electrochemical determination method for puerarin in Radix Puerariae. Methods The electrochemical behavior and content determination of puerarin were investigated by using coulomb array detector. Results In phosphate buffer with PH 4.32, puerarin showed good linearity in the range of 0. 16 ng - 162 ng. The regression equation was A = 1444.7C ＋ 88. 635, r = 0. 9998 （ n = 7）, the average recovery was 96.88 %, and RSD was 0. 08 %. Conclusion This method is simple and rapid, and the result is accurate and reliable. It can be used for the determination of puerarin content and biological samples.

关 键 词：葛根素 含量测定 电化学检测法 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]