HPLC法测定益视颗粒中大黄素的含量  

Determination of Emodin in Yishi Granules by HPLC

作　　者：范艳冰[1] 丁群彬[2] 

机构地区：[1]广州医学院羊城医院,广州510140 [2]广州中医药大学第一附属医院,广州510405

出　　处：《中药新药与临床药理》2009年第2期158-160,共3页Traditional Chinese Drug Research and Clinical Pharmacology

摘　　要：目的建立高效液相色谱法测定益视颗粒中大黄素的含量。方法采用高效液相色谱法,色谱柱为AlltechC18柱(250mm×4.6mm,5μm);流动相:甲醇-0.1%磷酸(80:20);流速:1mL·min-1;检测波长:254nm;柱温:30℃。结果大黄素分离良好,在32.8～131.2μg范围内与峰面积线性关系良好,r=0.9999,平均回收率为100.2%,RSD=1.52%(n=6)。结论该测定方法简便可行、重复性好,可用于该制剂中有效成分大黄素的含量测定。Objective To establish a method of HPLC assay for determining emodin in Yishi Granules. Methods The effeetive components in Yishi Granules were determined by HPLC. The chromatographic conditions were as follows： Alhech C18 eolumn （250 mm ×4.60 mm, 5 μm）, mobile phase consisting of methanol -0. 1% phosphoric acid （80 ： 20）, flow rate at 1 mL · min- 1, detecting wavelength being 254 nm, and the column temperature being 30 ℃. Results The separation and the linearity were good in the range of 32.8 - 131.2 μg, r =0. 999 9. The average reeovery of emodin was 100.20 % , and RSD = 1.52 % （ n = 6）. Conclusion The quantitative method for determining the active eomponents of Yishi Granules is simple, feasible and reprodueible, and is beneficial for the quality control of Yishi Granules.

关 键 词：益视颗粒 大黄素 高效液相色谱法 含量测定 

分 类 号：R284.1[医药卫生—中药学]