滋肾宁神丸中二苯乙烯苷的含量测定及其影响因素初探  

Determination of Stilbene Glucoside in Zishen Ningshen Pills by HPLC and Preliminary Study on Its Influence Factors

作　　者：张惜强 林荣锋[2] 何耀慧[2] 陈剑平[2] 黄晓其[2] 施旭光[2] 苏子仁[2] 

机构地区：[1]广东宏兴制药厂,广东潮州521011 [2]广州中医药大学,广州510405

出　　处：《中药新药与临床药理》2009年第2期163-165,共3页Traditional Chinese Drug Research and Clinical Pharmacology

摘　　要：目的建立滋肾宁神丸中二苯乙烯苷的含量测定方法,考察不同工艺因素对其含量的影响。方法采用HPLC法测定滋肾宁神丸中二苯乙烯苷含量,按照滋肾宁神丸制剂工艺的过程制备模拟的中间产物,用HPLC法测定各中间产物中二苯乙烯苷含量并计算其保留率和转移率。结果滋肾宁神丸中二苯乙烯苷达到良好分离,在测定范围内线性良好,回收率在98%～102%之间,烘干粉末的二苯乙烯苷保留率比原药材粉末低60.3%。结论所建立的定量方法简便可行、重复性好,可用于滋肾宁神丸的质量监控;现有的常压干燥是导致二苯乙烯苷含量降低的主要因素,可考虑以减压干燥方法替代常压干燥方法。Objective To establish a method of HPLC assay for determining stilbene glucoside in Zishen Ningshen Pills （ZNP）, and to study the influence factors on the content of stilbene glucoside in the process of preparation. Methods HPLC was used for the determination of stilbene glucoside in ZNP. Through simulation the process of preparation, the stilbene glueoside content in the intermediate products was determined by HPLC, and its retention rate and metastasis rate were also investigated. Results The resolution and the linearity of stilbene glucoside were fine, the average recoveries being 98 % - 102 %. The retention rate of stilbene glucoside in the drying powder was 60.3 % , lower than that in the original medicinal powder. Conclusion The quan- titative method for determining the ingredients in ZNP is simple, feasible and reproducible, and is beneficial for quality control of ZNP. The drying process under normal pressure is the main influence factors of the decrease of stilbene glucoside content, and the decompression drying can be taken into account to take the place of the atmospheric drying.

关 键 词：二苯乙烯苷 滋肾宁神丸 含量测定 HPLC 

分 类 号：R284.1[医药卫生—中药学] R284.2[医药卫生—中医学]