白英水提物诱导人胃癌SGC-7901细胞凋亡的实验研究  

Study on Apoptosis of Human Stomach SGC-7901 Cells Induced by Extracts of Solanum lyratum

作　　者：万福生[1] 吴剑[1] 李华[1] 涂硕[1] 余乐涵[1] 

机构地区：[1]南昌大学医学院生物化学与分子生物学教研室,江西南昌330006

出　　处：《中药材》2009年第2期245-249,共5页Journal of Chinese Medicinal Materials

基　　金：江西省科技攻关重点项目(060004)

摘　　要：目的:观察白英水提物(ESL)对胃癌SGC-7901细胞凋亡及相关基因表达的影响。方法:常规法制备ESL;实验分正常对照组、白英处理组和顺铂处理组。白英处理组加入ESL终浓度分别为12.5、25、50 mg/mL。MTT法检测细胞增殖抑制率,荧光显微镜观察SGC-7901细胞凋亡及形态变化,半定量RT-PCR分析基因bcl-xl、bid、Caspase-9 mRNA表达,荧光分析法测定Caspase-3活性。结果:与正常对照组比较,三个剂量白英处理组SGC-7901细胞增殖抑制均有显著性增加,且呈明显的时效和量效关系,细胞凋亡显著增多(P<0.05),表现细胞核固缩、染色质凝集、边聚等;bcl-xl mRNA表达显著下降(P<0.05),bid、Caspase-9 mRNA表达显著性升高(P<0.05),并随ESL浓度增加而加强,Caspaes-3活性亦显著性增高(P<0.01)。结论:ESL能剂量依赖性诱导SGC-7901细胞凋亡和抑制增殖,其作用与调控bcl-xl、bid、Caspase-9 mRNA表达和增强Caspase-3活性相关。Objective ：To investigate the effects of extracts of Solanum lyratum （ESL） on the apoptosis of Human stomach cancer SGC-7901 cells. Methods：Dried whole herbs of Solanum lyratum were extracted by boiling distilled water. SGC-7901 Cells were randomly divided into control group, ESL-treated groups （ 12. 5 g/L,25 g/L,50 g/L ） and the positive control（25 mg/L DDP） group. The growth inhibitory rate was evaluated by MTT assay. Morphological changes of apoptosis were observed with fluorescence microscope. Cell apoptosis rate was determined by flow cytometry. Expressions of bcl-xl, Caspase-9 and bid mRNA were detected by semi-quantitive RT-PCR. The activity of Caspase-3 was detected by Fluorospectrophotometry. Results：Compared with control group,the cell proliferation inhibitory rate and apoptosis rate of Human stomach cancer SGC-7901 cells increased obviously （P 〈 0. 05 ）. There were obvious changes of morphology of the SGC-7901 cells as the nuclear shrinkage,chromatin condensation and margination;The expression of bcl-xl mRNA decreased obviously（ P 〈 0. 05 ）, the expression of Caspase-9 and bid mRNA increased obviously respectively （ P 〈 0. 05 ）, and displayed effect in a dose-dependent manner in the SGC-7901 cells of the ESL-treated groups. The activity of Caspase-3 in the SGC-7901 cells of the ESL-treated groups were higher than that of the control group significantly（P 〈 0. 01 ）. Conclusion：ESL can induce apoptosis and inhibit proliferation of the Human stomach cancer SGC-7901 cells by regulating expression of bcl-xl, Caspase-9 and bid genes and strengthening the activity of Caspase-3.

关 键 词：白英 凋亡 基因表达 SGC-7901细胞 

分 类 号：R285.5[医药卫生—中药学]