酶解-溶剂联合提取银杏黄酮的工艺研究  

Study on Enzyme-Solvent Extraction Process of Flavonoids from Leaves of Ginkgo biloba

作　　者：欧阳娜娜[1] 李湘洲[1] 罗正[1] 

机构地区：[1]中南林业科技大学材料科学与工程学院,湖南长沙410004

出　　处：《中药材》2009年第2期279-283,共5页Journal of Chinese Medicinal Materials

基　　金：国家科技部"十一.五"科技支撑项目(2006BAD18B0404);湖南省科技厅科技攻关项目(05NK3056);中南林业科技大学校青年基金项目(07029B)

摘　　要：目的:研究酶解与溶剂联合提取银杏叶中黄酮类化合物的最佳工艺条件。方法:以黄酮得率为考察指标,通过单因素试验及正交实验确定最佳提取工艺。结果:酶解-溶剂联合提取银杏黄酮的最佳工艺条件是:酶解pH值为5.7,酶解浓度为0.4 mg/mL,酶解温度为45℃,酶解时间为2h,液固比为20∶1,提取温度为70℃,提取时间为2h,乙醇浓度为70%,银杏黄酮平均得率为3.62%。结论:采用酶解-溶剂联合提取银杏黄酮,工艺方法简单,可大大提高原料利用率。Objective ： To study the optimum enzyme-solvent extraction conditions of flavonoids from leaves of Ginkgo biloba. Methods：To optimize the process by single factor experiments and orthogonal test according to the yield of flavonoids. Results：The optimum extraction conditions were established as follows ： pH5. 7, the concentration of enzyme 0. 4 mg/mL, enzymatic hydrolysis temperature 45℃, enzymatic hydrolysis time 2h, the rate of liquid to material 20： 1, extraction temperature 70℃, extraction time 2h, the concentration of ethanol 70% ,the average yield of flavonoids from leaves of Ginkgo biloba was 3.62%. Conclusion： The method is simple and can increase material utility effectively.

关 键 词：银杏叶 黄酮 酶解-溶剂提取 

分 类 号：R284.2[医药卫生—中药学]