CellSense生物传感器毒性分析系统应用研究  

The Application Performance of CellSense Biosensor for Toxicity Assessment

作　　者：贺莹[1] 王学江[1] 王虹[1] 赵红宁[1] 赵建夫[1] 夏四清[1] 

机构地区：[1]同济大学环境科学与工程学院污染控制与资源化研究国家重点实验室,上海200092

出　　处：《生态毒理学报》2009年第1期108-113,共6页Asian Journal of Ecotoxicology

基　　金：国家自然科学基金项目(No.20707014);教育部留学回国人员科研启动基金

摘　　要：介绍了一种基于大肠杆菌(Escherichia coli)的CellSense生物传感器毒性分析系统,对其毒性分析条件进行了初步优化,并探讨了其在重金属离子生物急性毒性分析中的应用性能.结果表明,以p-苯醌为电子传递介质时,基于大肠杆菌的CellSense生物传感器具有良好的毒性分析特性,测试得Hg2+、Cu2+、Zn2+和Ni2+四种金属离子的EC50分别为1.1、6.5、66.0和119.0μg·mL-1;呼吸基质的pH低于6.0和盐度(NaCl)高于3%(w/w)时,对E.coli的活性产生一定的抑制作用.A cost-effective strategy using an amperometric biosensor with Escherichia coli （E. coli ） that provides a rapid toxicity determination of toxicants is described. The Cellsense biosensor system comprises a biological component immobilized in intimate contact with a transducer which converts the biochemical signal into a quantifiable electrical signal. It is showed that CeUSense biosensor with E. coli has a good performance when p-benzoquinone is chosen as redox mediator. The 50% effective concentrations （EC50）values of Hg^2＋, Cu^2＋, Zn^2＋ and Ni^2＋ are 1.1, 6.5, 66.0 and 119.0μg.mL^-1, respectively, which are in accordance with the results of conventional bacterial counting method. When the respiratory substrates pH is lower than about 6.0 and salinity（NaCl） is higher than 3%（w/w）, the activity of E. coli is inhibited significantly.

关 键 词：微生物传感器 介质 毒性 重金属 

分 类 号：X853[环境科学与工程—环境工程] X132