2,3,7,8-四氯二苯并二噁英和β-萘黄酮暴露对斑马鱼肝和鳃MROD酶活性的影响  

Studies on Hepatic and Branchial MROD Activities in Zebrafish Exposed to 2,3,7,8-Tetrachlorodibenzo-p-Dioxin and Beta-Naphthoflavone

作　　者：谢英明[1] 聂芳红[2] 汤陈坚[3] 杨蓉[1] 林红英[1] 陈进军[1] 

机构地区：[1]广东海洋大学动物医学系,湛江524088 [2]广东海洋大学食品科技学院,湛江524088 [3]湛江教育学院生物系,湛江524037

出　　处：《生态毒理学报》2009年第1期142-146,共5页Asian Journal of Ecotoxicology

基　　金：广东省国际合作项目(No.2007B050200023);广东省自然科学基金项目(No.05011789)

摘　　要：为了研究2,3,7,8-四氯二苯并二噁英(TCDD)和β-萘黄酮(β-NF)对斑马鱼(Danio rerio)肝脏和鳃CYP1A依赖性7-甲氧基-3-异酚噁唑酮-脱甲基酶(MROD)活性的影响,分别利用不同浓度的TCDD(0、0.05、0.1、0.2、0.4μg·L-1)和β-NF(0、25、50、100、200μg·L-1)对斑马鱼进行水浴暴露,48h后取样测定肝脏和腮MROD活性.结果表明,与对照组比较,TCDD暴露组和β-NF暴露组斑马鱼肝脏和鳃MROD活性均显著增强(p<0.01),且MROD活性的增加与TCDD或β-NF暴露浓度呈现明显的剂量-效应关系.初步推断斑马鱼肝脏和鳃CYP1A依赖性MROD酶活性可能能够作为TCDD或β-NF污染的生物标志物.In order to investigate the effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin（TCDD）and beta-naphthoflavone （β- NF ）on hepatic and branchial CYP1A dependent-methoxyresorufin-O-demethylase （ MROD ） activities in fish, zebrafish （ Danio re rio ）was exposed to different concentrations of TCDD （0, 0.05, 0.1, 0.2 and 0.4μg. L^-1 ）or β-NF （0, 25, 50, 100 and 200μg.L^-1） for 48h, and then hepatic and branchial MROD activities were measured. Results showed that the hepatic and branchial MROD activities significantly （p〈0.01）increased when zebrafish were exposed to TCDD or β-NF. There were dose-effect relationships between the concentration of chemicals and MROD activities. Preliminary analysis showed that the induction of hepatic and branchial CYP1A dependent-MROD activities in zebrafish might be taken as a biomarker for environmental exposure to TCDD or β-NF.

关 键 词：2 3 7 8-四氯二苯并二噁英 β-萘黄酮 7-甲氧基-3-异酚噁唑酮-脱甲基酶 生物标志物 斑马鱼 

分 类 号：S859.83[农业科学—临床兽医学] R994.6[农业科学—兽医学]