蜕皮甾酮对肿瘤坏死因子致人脐静脉内皮细胞损伤的影响  

Effects of ecdysterone on human umbilical vein endothelial cells injuried by tumor necrosis factor

作　　者：吴旭[1] 林水金[1] 杨映波 冯素珍[1] 

机构地区：[1]第三军医大学大坪医院野战外科研究所

出　　处：《中国病理生理杂志》1998年第1期58-62,共5页Chinese Journal of Pathophysiology

摘　　要：目的和方法：实验以培养的人脐静脉内皮细胞（ＨＵＶＥＣ）为研究对象，应用大剂量肿瘤坏死因子（ＴＮＦ）造成ＨＵＶＥＣ损伤，观察培养上清液血管紧张素转换酶（ＡＣＥ）及ＨＵＶＥＣ的超微结构变化，研究蜕皮甾酮（ＥＤＳ）对内皮细胞损伤的影响。结果：大剂量ＴＮＦ作用于ＨＵＶＥＣ时，其培养上清液的ＡＣＥ非常明显增高，超微结构破坏；应用ＥＤＳ，则培养上清液的ＡＣＥ有所减少、超微结构的损害程度有所减轻。结论：ＥＤＳ能够减轻人血管内皮细胞的损伤，揭示了ＥＤＳ在抗血管内皮细胞损伤方面的价值。AIM: To study effects of ecdysterone(EDS) on injuried vascular endothelial cells. METHODS:The vascular endothelial cells were damaged by recombinant human tumor necrosis factor α (rh-TNFα).The experiments were designed to observe changes of angiotensin converting enzyme(ACE) in culture liquid of human umbilical vein endothelial cells (HUVEC) and changes of ultrastructure of HUVEC.RESULTS: ACE activity in culture liquid of HUVEC distinctly raised and ultrastructure of HUVEC were destroied when HUVEC were injuried by a large dosage of rh-TNFα,but when EDS were applied in injuried HUVEC, ACE activity in culture liquid of HUVEC reduced and the damages of ultrastructure of HUVEC allevated. CONCLUSION:EDS was able to attenuate the injury of human vascular endothelial cells . This work revealed the value of EDS on treatment of injuried vascular endothelial cells. .

关 键 词：血管 内皮细胞损伤 肿瘤坏死因子 蜕皮甾酮 

分 类 号：R654.3[医药卫生—外科学] R361[医药卫生—临床医学]