采用全菌抗原干燥包被法ELISA检测禽霍乱血清抗体的研究  

Whole Cell Dry-coating in ELISA for Detecting Serum Antibody against Fowl Cholera

作　　者：徐步[1] 高明燕[1] 龚建森[1] 吕晓娟[1] 单艳菊[1] 刘学贤[1] 

机构地区：[1]中国农业科学院家禽研究所,江苏扬州225003

出　　处：《中国家禽》2009年第5期30-33,共4页China Poultry

基　　金：江苏省"十一五"农业科技攻关项目(BE2006363);中国农科院家禽研究所青年科研项目(S07007)

摘　　要：利用戊二醛的偶联作用将禽多杀性巴氏杆菌的全细胞抗原在聚苯乙烯酶标板上进行干燥包被,酶标板以2.5%的戊二醛溶液预处理。通过直接和间接ELISA方阵滴定确定其包被的全菌抗原最适浓度为108个菌/mL。特异性、重复性、稳定性检验,以及初步建立的间接ELISA用于样品检测的结果表明,所创建的禽多杀性巴氏杆菌全菌抗原干燥包被方法能够很好地应用于建立检测禽霍乱血清抗体的ELISA。Pasteurella multocida was integrally dry-coated on the basis of glutaraldehyde coupling of the whole cell antigen to microliter plates. Glutaraldehyde of 2.5% concentration was used for pretreatment of ELISA plates. Using the method of chessboard titration,a hundred million of whole cells per milliliter in PBS buffer were selected for the proper dry-coating concentration. Specificity,repeatability and stability examination＇s result as well as the preliminary indirect ELISA used in the sample test indicated that the whole cell dry-coating could be rather helpful to development of ELISA detecting fowl cholera antibody.

关 键 词：多杀性巴氏杆菌 ELISA 抗原包被 

分 类 号：S858.28[农业科学—临床兽医学] S858.285.3[农业科学—兽医学]