消瘕方抑制人乳腺癌裸鼠移植瘤血管内皮生长因子表达的实验研究  

Effects of Xiaojia Decoction on Expression of Vascular Endothelial Growth Factor in Transplantation Tumors of Breast Cancer in Nude Mice

作　　者：欧阳华强[1] 黄雯霞[1] 宋明志[1] 刘鲁明[1] 沈晔华[1] 罗建民[1] 

机构地区：[1]复旦大学附属肿瘤医院,复旦大学上海医学院肿瘤学系,上海200032

出　　处：《中国实验方剂学杂志》2009年第3期44-47,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：上海市卫生局中医药科研基金项目(2004Y003A)

摘　　要：目的:研究消瘕方对人乳腺癌MDA-MB-435细胞株裸小鼠移植瘤中血管内皮生长因子(VEGF)表达的影响。方法:以体外培养的人乳腺癌MDA-MB-435细胞接种于裸小鼠右腋部位制造裸鼠皮下移植瘤模型,随机分为生理盐水组、5-氟尿嘧啶组及消瘕方小,中,大剂量组,每组小鼠予以相应药物治疗6周后脱颈处死,称瘤重,计算肿瘤生长抑制率,并采用RT-PCR法、免疫组化法检测肿瘤组织中VEGF mRNA及蛋白的表达。结果:消瘕方可抑制乳腺移植瘤生长,降低瘤体内VEGF mRNA表达(P<0.05),下调相应VEGF蛋白表达(P<0.05),其中以中剂量组效果更明显。结论:消瘕方的抑瘤作用与其下调瘤体内VEGF表达密切相关。Objective： To study the effects of Xiaojia Decoction on expression of Vascular Endothelial Growth Factor in transplantation tumors of the breast cancer cell line MDA-MB-435 in nude mice. Methods： The MDA-MB-435 human breast cancer cells cultured in vitro were subcutaneously inoculated into BALB/c nude mice to establish the transplanted tumor model. The mice were then randomized into five groups and treated with Xiaojia Decoction or other remedies for 6 weeks. Then we sacrificed the mice and weighed the tumor, calculated the inhibitory rate of tumor weight, the expression of VEGF on mRNA and protein level was determined by means of RT-PCR and immunohistochemical assay. Results： Xiaojia Decoction could inhibit the growth of tumor conspicuously, lower the expression of both mRNA and protein level of VEGF （P 〈 0.05）, especially for the medium-dose group. Conclusion： This Decoction＇ s effects on down-regulating VEGF have close relation with its function of inhibiting the tumor growth.

关 键 词：乳腺肿瘤 消瘕方 血管内皮生长因子 

分 类 号：R285.5[医药卫生—中药学]