西藏管花秦艽花弱极性成分的GC-MS分析  

Identification of Puny Polar Components of the Flower of Gentiana siphonantha in Tibet by GC-MS

作　　者：沈杰[1] 叶蕴华[1] 张秀[1] 周亚伟 

机构地区：[1]北京大学化学与分子工程学院,北京分子科学国家实验室,生物有机与分子工程教育部重点实验室,北京100871 [2]北大世佳研究中心,北京100084

出　　处：《北京大学学报（自然科学版）》2009年第2期365-370,共6页Acta Scientiarum Naturalium Universitatis Pekinensis

摘　　要：首次对西藏管花秦艽干燥花的弱极性成分进行气相色谱-质谱分析。用乙醇回流提取管花秦艽的干燥花,得到浸膏后,以不同沸程的石油醚萃取出其弱极性成分,然后应用GC-MS分析技术,对石油醚萃取物进行化学成分分析研究和比较。用石油醚萃取物直接进行GC-MS分析,除检测到烷烃外,发现60～90℃石油醚萃取物中存在含量较高的4种芳香酯类化合物。两种石油醚萃取物经甲酯化处理后,共鉴定了30种弱极性成分,主要为脂肪酸酯类和烷烃类。管花秦艽花含量最高的脂肪酸是14-甲基-十五烷酸、亚麻酸和亚油酸。实验结果为了解管花秦艽的化学物质基础和进一步开发研究提供了一定的科学依据。Puny polar components of Gentiana siphonantha are analyzed by GC-MS for the first time. The dried flower of G. siphonantha is extracted by the reflux of ethanol ; the residue is suspended in water and extracted successively with different boiling range petroleum ether respectively. Alkanes are main component in two different extractions before esterification detected by GC- MS,and four aromatic esters exist with the high content in the petroleum ether （60-90~C） fractions before esterification. Thirty compounds are identified from two different etherifications by GC-MS. Fatty acids are one of the main components, which the contents of 14-methyl-pentadecanoic acid, 9, 12-octadecadienoic acid and 9, 12, 15-octadecatrienoic acid are high. The results would provide important foundation for understanding the constituents and further exploitation of Gentiana siphonantha.

关 键 词：西藏管花秦艽 弱极性成分 气相色谱/质谱联用 

分 类 号：R284[医药卫生—中药学]