环磷腺苷葡胺和氨茶碱对小鼠急性缺氧性脑损伤的保护作用  

Protective effect of combined MCA and aminophylline on hypoxic brain injury of mice.

作　　者：杨峰霞[1] 杨明[1] 姚英民[1] 

机构地区：[1]南方医科大学南方医院新生儿科,广东广州510515

出　　处：《中国优生与遗传杂志》2009年第3期25-26,108,共3页Chinese Journal of Birth Health & Heredity

摘　　要：目的观察环磷腺苷葡氨(MCA)和氨茶碱联用对小鼠急性缺氧性脑损伤(HBI)的干预作用,初步探讨其可能的神经保护机制。方法90只小鼠分为正常对照组、HBI组、氨茶碱组、MCA组、两药合用组。缺氧后72h测各组脑组织含水量、超氧化物歧化酶活性(SOD)及氧化代谢产物丙二醛(MDA)含量;HE染色观察各组海马区结构及神经细胞的调亡情况。结果与HBI模型组相比,药物干预组脑组织含水量、MDA下降,SOD升高,病理变化减轻,细胞调亡减少,病理变化尤以两药合用组改善最显著。结论两药联用可有效抑制缺氧后脑组织内氧自由基生成,减轻脑水肿及神经细胞的调亡。Objective ： To study the therapeutic effect of combined administration of MCA and Aminophylline on the hypoxic brain injury （HBI） mouse model and the relevant involving mechanisms. Methods： A total of 90 mouses were randomly divided into： normal control group, HBI group, Aminophylline group, MCAgroup and MCA ＋ Aminophylline group. 72h after hypoxia the brains were taken out and the water content and the level of SOD, MDA were measured ; HE staining employed to study the morphological changes in the hippoeampal neuron after treatment. Results： Compared with HBI group, the content of water and the level of MDA were markedly reduced （ P 〈 0.05, 0.01 ） , while the levels of SOD were increased significantly （ P 〈 0. 01 ）. Hippocampal neuron pathological changes reduced in the drug groups, especially inMCA ＋ Aminophylline group. Conclusion： The combined administration of MCA and Aminophylline may decrease the content of water and the level of MDA, increase the level of SOD, and inhibit neuronal apoptosis in hippoeampus.

关 键 词：缺氧性脑损伤 环磷腺苷葡胺 氨茶碱 小鼠 

分 类 号：R96[医药卫生—药理学]