3种方法对日本血吸虫感染家兔的疗效评价  

Comparasive study on three methods in evaluation of curative effect on Schistosoma japonicum infection in rabbits

作　　者：陆正贤[1] 李松华[1] 夏超明[2] 荣荣 时长军 

机构地区：[1]江苏省启东市人民医院,226200 [2]苏州大学医学院病原生物学教研室 [3]江苏省苏州新力生技术研究所

出　　处：《检验医学与临床》2009年第6期401-403,共3页Laboratory Medicine and Clinic

摘　　要：目的用聚合酶链反应(PCR)、酶联免疫吸附试验(ELISA)和环卵沉淀试验(COPT)3种方法评价吡喹酮治疗日本血吸虫感染家兔的疗效。方法家兔感染日本血吸虫尾蚴后7周和8周,分别给予吡喹酮治疗,治疗前后每周采血1次,分别用3种方法检测感染家兔治疗前后的血清DNA、IgG和环卵沉淀率(COPR)水平。结果ELISA检测法在动物模型治疗6个月后,其血清抗体仍维持在一定水平;COPT检测法于治疗后5个半月在家兔血清中找不到阳性虫卵;PCR法在治疗后3个月的家兔血清中未扩增出DNA。结论PCR法的疗效考核价值优于常规ELISA法和COPT法。Objective To compare the advantages and disadvantages of three methods,polymerase chain reaction （PCR）,enzyme linked immunosorbent assay （ELISA） and eircum oval precipitation test （COPT）,in evaluation of curative effect on Sehistosoma japonieum infection in rabbits. Methods The rabbits were infected by Sehistosoma japonicum cerearie. Seven and eight weeks later,the rabbits underwent pyquiton therapy. The venous blood was collected and measured weekly before and after treatment. Three different methods （PCR,ELISA and COPT） were applied to measuring serum DNA,IgG and eireum oval precipitation rate （COPR）. Results ELISA： the sera IgG kept a high level in six months after treatment;COPT： in five and a half months,no positive egg was found in sera of the rabbits;PCR： no DNA amplication occurred in the sera in three months after treatment. Conelusion The evaluation value of PCR on therapeutic effect outstrips that of routine ELISA and COPT.

关 键 词：日本血吸虫 聚合酶链反应 酶联免疫吸附试验 环卵沉淀试验 评价研究 

分 类 号：R532.21[医药卫生—内科学] R446.61[医药卫生—临床医学]