灰飞虱对毒死蜱的抗性风险评估、抗性遗传及交互抗性  

Risk Assessment and Inheritance Mode to Chlorpyrifos in Laodelphax striatellus (Fallén) and Cross-resistance to Other Insecticides

作　　者：王利华[1] 刘艳荷[1] 方继朝[1] 

机构地区：[1]江苏省农业科学院植物保护研究所,江苏南京210014

出　　处：《江苏农业学报》2009年第1期73-78,共6页Jiangsu Journal of Agricultural Sciences

基　　金：江苏省博士后科研资助计划项目(0702012C);江苏省重大攻关项目(BE2006303);江苏省农业科技自主创新资金重点项目[CX(08)606]

摘　　要：采用群体筛选法对2001年采自云南楚雄对毒死蜱敏感的灰飞虱（SS）进行连续筛选，筛选13代后，获得抗性为107．86倍的抗性（RR）品系。估计其抗性现实遗传力为0．33，在室内同等选择条件下，致死率为50％-90％时，抗性上升10．00倍需4-9代。利用RR品系与敏感品系ss进行遗传杂交分析灰飞虱对毒死蜱的抗性遗传规律，结果表明正、反交后代对毒死蜱的抗性分别为12．96倍和12．78倍，显性度D值分别为0．095和0．089，说明灰飞虱对毒死蜱的抗性为常染色体遗传，主效基因为不完全显性。通过分析杂交F．代和敏感品系SS回交后代对毒死蜱的LD-P线，表明灰飞虱对毒死蜱的抗性为单基因性状。交互抗性谱测定结果表明，RR品系对有机磷杀虫剂敌敌畏有5．82倍的交互抗性，对烟碱类噻虫嗪、苯基吡唑类氟虫腈和大环内酯类阿维菌素没有交互抗性。The small brown planthopper, Laodelphax striatellus （Fallen） collected from Chuxiong, Yunnan Province, was continuously selected in greenhouse for resistance to chlorpyrifos. After 13 generations, the resistance had increased to 107.86-fold, the realized heritability of resistance was 0. 33. Theoretically, to obtain a 10. 00-fold resistance requires 4-9 generations under selection pressure of 50% -90% mortality for each selection generation. The inheritance mode of resistance to chlorpyrifos was evaluated using genetic crosses between resistant and susceptible strains in the laboratory. Resistance to chlorpyrifos of Ft hybrids from reciprocal crosses was 12. 96-fold and 12. 78-fold respectively, and the value of D was 0. 095 and 0. 089 respectively, which indicated that the chlorpyrifos resistance was not sex linked and incompletely dominant. Analysis of the LD-P line to chlorpyrifos of the backcross between F1 hybrids and SS showed that the chlorpyrifos resistance in the small brown hopper was controlled by a single gene locus. The results in cross-resistance experiments showed RR strain had significant cross-resistance to dichlorvos, but not to fipronil, abamectin and thiamethoxam.

关 键 词：毒死蜱 现实遗传力 抗性遗传 交互抗性 

分 类 号：S433.3[农业科学—农业昆虫与害虫防治] S481.4[农业科学—植物保护]