江苏3个秀丽白虾野生群体遗传多样性的AFLP分析  

Genetic Diversity of Three Wild Exopalaemon modestus Populations from Jiangsu Province Revealed by AFLP Markers

作　　者：许志强[1] 葛家春[1] 曹廷[2] 李跃华[1] 潘建林[1] 

机构地区：[1]江苏省淡水水产研究所,江苏南京210017 [2]南京大学生命科学学院,江苏南京210093

出　　处：《江苏农业学报》2009年第1期160-164,共5页Jiangsu Journal of Agricultural Sciences

基　　金：江苏省科技厅公益研究与服务专项(BM2005706);江苏省科研条件与设备专项(BM2006503);农业部农业科技跨越计划青虾优良品系的规模化繁育和产业化示范

摘　　要：采用AFLP分子标记技术对江苏省内3个秀丽白虾野生群体的遗传多样性水平及遗传结构进行了分析。采用3个引物组合（E-AAC／M-CAA、E-AAC／M-CAG、E-AAC／M-CAT），共扩增出102个清晰位点，其中93个为多态性位点。结果显示，3个秀丽白虾野生群体在物种水平和群体水平上均蕴含着较为丰富的遗传多样性。在物种水平上，秀丽白虾多态性位点百分率（PPL）为91．18％，Nei’s基因多样性指数（h）为0．391，Shannon信息指数（I）为0．562。群体水平上的PPL为58．82％-70．59％，平均为65．4％，其中骆马湖群体最高，为70．6％；长江群体最低，为58．8％。群体间Nei’S多样性指数为0．268-0．320，平均为0．292；Shannon信息指数为0．381-0．454，平均为0．417。长江南京段群体与太湖群体间的分化系数较低（0．141），长江南京段群体与骆马湖群体间的遗传分化系数较高（0．234）。建议加强秀丽白虾引种、育种工作中的管理力度，建立详实的引育种档案，推动秀丽白虾养殖工作的发展。The genetic diversity and genetic structure of three wild populations of the freshwater prawn Exopalaemon modestu~ in Jiangsu Province were investigated using AFLP markers. A total of 102 unambiguous polymorphic bands were detected with three primers combinations （E-AAC/M-CAA, E-AAC/M-CAG,E-AAC/M-CAT） , of which 93 were polymorphic loci. A fairly high genetic diversity was found at the level of species and among the populations. At the level of species, the percentage of polymorphic loci was 91.18% , Nei＇s gene diversity index（h） was 0. 391 and Shannon information index（I） was 0. 562. The percentage of polymorphic loci of 3 populations ranged from 58. 82% to 70. 59% , with average of 65.4%. The population with the highest percentage （70. 6% ） of polymorphic loci was found in the Luomahu Lake, and the population with the lowest percentage polymorphism （33.64%） was the one collected from Yangtze River in Nanjing. Nei＇s gene diversity of the 3 populations was from 0. 268 to 0. 320, with average of 0. 292. The Shannon information index was from 0. 381 to 0. 454, with average of 0. 417. The genetic differentiation between the population of Yangtze River in Nanjing and the population of Tai Lake was lower than other pair of populations. It was suggested that effective measures should be taken to avoid the degeneration of source germplasm in the reproduction and breeding of the prawn.

关 键 词：秀丽白虾 AFLP 遗传多样性 

分 类 号：S966.12[农业科学—水产养殖]