抗真菌三价载体转化辣椒的研究  

Transformation of A Trivalent Antifungal Recombinant into Pepper (Capsicum annuum L.)

作　　者：敬国兴[1,2] 曾富华[1,2] 李伏钦[1] 陈永双[1] 何永敏[1] 

机构地区：[1]湛江师范学院生命科学与技术学院,广东湛江524048 [2]湖南农业大学生物与发酵工程实验室,湖南长沙410128

出　　处：《江苏农业学报》2009年第1期165-168,共4页Jiangsu Journal of Agricultural Sciences

基　　金：广东省科技计划项目(Z002A2070402);广东省自然科学基金项目(51011730)

摘　　要：利用农杆菌介导法将抗真菌蛋白的葡聚糖酶(Glu)基因、几丁质酶(Chi)基因和萝卜抗真菌蛋白(Rs-AFP2)基因构建的三价表达载体(GCR)转化辣椒,经PCR、PCR-Southern blotting分子检测证实,外源基因已经整合到辣椒植株基因组DNA中。对辣椒离体子叶进行辣椒疫病病菌抗病性检测,证明转基因辣椒离体子叶对疫病有很强的抗性。The trivalent expression vectors containing chitinase （ Chi ）/β-1,3- Glucanase （Glu） / rahpanussativus-antifungal protein （Rs-AFP2） genes were transferred into peppers through agrobacterium-mediated way. It was confirmed that these genes were integrated into the peppers genome by PCR and PCR-Southern blotting. Fungal growth of Phytophthora capsici Leonian was strongly inhibited on the leaf of transgenic pepper, which indicated the expression of resistance to phytophthora capsici in transgenic pepper.

关 键 词：辣椒 三价表达载体 转化 抗病 

分 类 号：S641.3[农业科学—蔬菜学]