铈(Ⅳ)-亚硫酸钠体系流动注射化学发光法测定叶酸  

Determination of folic acid by flow-injection chemiluminescence using cerium(Ⅳ) sulfate-sodium sulfite system

作　　者：蔡卓[1] 江彩英[1] 赵静[1] 李玉娥[1] 莫丽君[1] 

机构地区：[1]广西大学化学化工学院,广西南宁530004

出　　处：《化学研究与应用》2009年第3期370-372,共3页Chemical Research and Application

基　　金：广西自然科学基金资助项目(0447008)

摘　　要：叶酸又称蝶酰谷氨酸，是一种维生素B族类的抗贫血药，尤适用于孕妇和婴儿红细胞性贫血。目前，测定叶酸的方法多为荧光法、高效液相色谱法、紫外分光光度法、电化学法、原子光谱法等。化学发光法测定叶酸的文献也曾有报道，它们分别采用的是鲁米诺体系和高锰酸钾体系，但这些方法的检出限比较高。A new flow-injection chemiluminescence method was developed for the determination of folic acid, based on the strong sensitization effect of folic acid on the ohemiluminescence（CL） reaction of Ce （ Ⅳ ）-Na2 SO3 in acidic medium. Under the optimum conditions,the linear relationship between the chemiluminescence intensity and the concentration of folic acid was over the range of 2.2 ×10^-6g ·L^-1 - 2.2 ×10^-4g ·L^-1 , and the detection limit was 1.7 ×10^-6g ·L^-1. Precision of the method was tested at the concentration level of 2.2 ×10^-4g ·L^-1 and the relative standard deviation （RSD） was 1.3% （n = 11 ）. The proposed method was successfully applied to the determination of folic acid in tablets. This method is accurate, sensitive and simple.

关 键 词：化学发光 流动注射 叶酸 铈(Ⅳ) 亚硫酸钠 

分 类 号：O657.3[理学—分析化学]