一种改良胚胎冻融法对冻融胚胎移植周期结局的影响  

Effect of modified embryo cryopreservation and thawing method on frozen-thawed embryo transfer cycles

作　　者：刁英[1] 谭兵兵[1] 杨智敏[1] 

机构地区：[1]遵义医学院第一附属医院生殖中心,贵州遵义563003

出　　处：《中国妇幼保健》2009年第7期942-944,共3页Maternal and Child Health Care of China

摘　　要：目的:探讨一种改良胚胎冻融法对冻融胚胎移植周期结局的影响。方法:回顾分析2003年1月～2008年6月,在本院生殖中心采用旧法冻融胚胎86个周期与改良法冻融胚胎95个周期胚胎的复苏存活率、胚胎完好率、周期妊娠率及胚胎种植率。结果:改良法冻融胚胎95个周期解冻胚胎248个,复苏存活率、胚胎完好率、周期妊娠率与胚胎种植率分别为93.1%、76.6%、38.9%和19.5%,均显著高于旧法冻融胚胎组(86个周期,解冻胚胎367个)的复苏存活率(53.4%)、胚胎完好率(12.8%)、周期妊娠率(20.9%)与胚胎种植率(9.7%),P<0.005。两种方法流产率差异无显著性(P>0.05)。结论:改良法冻融胚胎较旧法取得了更高的复苏胚胎存活率、胚胎完好率、周期妊娠率及胚胎种植率,达到国内较好中心水平,值得临床推广使用。Objective： To explore the effect of modified embryo cryopreservation and thawing method on frozen - thawed embryo transfer cycles. Methods： From January 2003 to June 2008, A retrospective study was conducted on 86 frozen - thawed embryo transfer cycles with old method and 95 cycles with the modified method. Then the post - thaw survival, blastomere fully intact rate, pregnanct rate, implantation rates of the two methods were compared. Results ： Among the 248 thawed embryos from 95 forzen - thawed embryo transfer cycles, the percentages of post - thaw survival （ 93.1% ）, 100% intact embryo （ 76. 6% ）, pregnancy （ 38.9% ） and implantation （ 19. 5% ） were significantly higher than those of old method （53.4%, 12.8%, 20. 9%, 9.7%, respectively） （P 〈0. 005） . There was no difference between the two methods in the miscarriage rate （ P 〉 0. 05 ） . Conclusion： Greater post - thaw embryo survival and 100% intact embryo rates were achieved, with a concomitant increasing in pregnancy and implantation rates by modifying the various steps in old method. The modified embryo cryopreservation and thawing method should be used in clinical practice widely.

关 键 词：胚胎冷冻 冻融胚胎移植 妊娠率 

分 类 号：R714.5[医药卫生—妇产科学]