不同胰酶消化方法用于制备猴胚肾细胞的评价  

Valuation of Trypsinization Methods Used for Preparation of Embryo kidney Cells

作　　者：吴炳元[1] 衡燮[1] 王燕[1] 李映波[1] 姜述德[1] 

机构地区：[1]中国医学科学院中国协和医科大学医学生物学研究所

出　　处：《云南大学学报（自然科学版）》1998年第2期95-96,共2页Journal of Yunnan University(Natural Sciences Edition)

摘　　要：为了提高猴胚肾细胞的产量和质量，对生物制品生产中常规使用的四种细胞制备方法进行实验性评价，结果表明热消化法和冷消化法较适合于猴胚肾细胞的制备，冷消化法又优于热消化法，细胞产量高稳定性好，细胞活力强，上述两种方法也适合于幼兔肾和胎猴肺细胞的制备．In order to increase the yield of embryo-kidney cell four kinds of trypsinization methods used in biological production were compared.The results showed that the heating (37℃) and cooling(4℃) trypsinization were suitable,but the cooling is higher and more stable than the heating for the yield of the cell harvest,the vigour of cells growth was also better.The young rabbit kidneys and embryo-monkey lungs were tested by using methods as mentioned above.

关 键 词：胰酶消化法 猴胚肾细胞 热消化 冷消化 细胞培养 

分 类 号：Q343.6[生物学—遗传学]