膜分离与沉淀剂结合提取腌肠衣盐水中肝素纳的工艺研究  

Craft study on the extraction of heparin sodium from pickle casing brine by the combination of membrane separation and precipitator

作　　者：李耀曾[1] 曹芸[1] 夏华[1] 姚世霞[1] 赵建邦[1] 杜兴[1] 张继萍 

机构地区：[1]甘肃省药品检验所,甘肃兰州730000 [2]陇盛肠衣厂,甘肃陇西748000

出　　处：《兰州大学学报（医学版）》2009年第1期68-70,75,共4页Journal of Lanzhou University（Medical Sciences）

摘　　要：目的探讨膜分离与沉淀剂结合提取腌肠衣盐水中肝素纳的工艺改进。方法采用盐解-离子交换-膜分离及蛋白沉淀除杂法从中提取肝素纳。结果从废弃的腌肠衣盐水中提取肝素钠取得了成功,与传统工艺(盐解-离子交换法)比较,洗脱液体积可浓缩至原体积的15%左右,乙醇用量可减少85%左右,而效价分别从传统工艺的96.82U/mg增加到157.58U/mg,总收率比传统工艺提高了12.5%。结论工艺方法可行、可靠,适宜于中、小型规模的生产。Objective To investigate the craft of extracting heparin sodium from pickle casing brine by the combination of membrane separation and precipitator. Methods Heparin sodium was extracted by salt solution-ionic exchange-membrane filtration and the protein precipitation method. Results The extraction of heparin sodium from pickle casing brine abandoned was succeeded. When compared with traditional craft （salt solution-ionic exchange method）, effluent volume could be condensed to 15% of the original physical volume, alcohol consumption could be reduced by 85% or so, but the titer was increased from 96.82 U/mg of traditional craft to 157.58 U/mg, and the total recovery rate was improved by 12.5%. Conclusion The method is feasible, the craft is credible and can be used for the production of small and medium-sized scales.

关 键 词：腌肠衣盐水 膜分离 沉淀剂 

分 类 号：R931.74[医药卫生—生药学]