银杏发根的转化及其悬浮培养无性系的建立  

TRANSFORMATION OF GINKGO HAIRY ROOT AND ESTABLISHMENT OF ITS SUSPENSION CULTURE CLONE

作　　者：刘树楠[1] 孙天恩[1] 李根保 

机构地区：[1]武汉大学分析测试科学系

出　　处：《武汉大学学报（自然科学版）》1998年第2期238-240,共3页Journal of Wuhan University(Natural Science Edition)

摘　　要：运用具有Ｒｉ质粒的发根农杆菌对银杏的叶片、幼芽和幼茎进行成功地转化，并建立银杏发根的悬浮培养无性系．冠瘿碱检测实验表明，发根农杆菌Ｒｉ质粒的Ｔ－ＤＮＡ部分已整合到银杏细胞中．这为利用生物技术开发银杏资源提供一条新的途径．In this thesis, Tender leaves,buds and stems of Ginkgo biloba L.were transformed successfully by Agrobacterium rhizogenes with Ri plasmid ,and its suspension culture clone was eastablished. The result of detection of opines suggested that A.rhizogenes had integrated its T DNA of the Ri plasmid into genomic DNA of ginkgo cells.A new way is offered to exploit ginkgo resources with biological technology.

关 键 词：银杏 发根农杆菌 发根 悬浮培养 无性系 

分 类 号：S792.950.1[农业科学—林木遗传育种]