金正日球根海棠叶片组织培养诱导的研究  

Study on Leaves of Kim Jong-il Begonia tuberhybrieda Vosa Induced by Tissue Culture

作　　者：张凤生[1] 樊绍翥[1] 薛丹丹[1] 姜淼[1] 李健虹[1] 王保菊[1] 武斌[1] 赵娜[1] 

机构地区：[1]哈尔滨市农业科学院,黑龙江哈尔滨150070

出　　处：《黑龙江农业科学》2009年第2期17-19,共3页Heilongjiang Agricultural Sciences

摘　　要：以金正日球根海棠（Begonia tuberhybrieda Vosa）的叶片为外植体进行组织培养的诱导，以Ms培养基为基本培养基，并附加不同浓度的细胞分裂素6-BA以及生长素NAA或2，4-D进行组织培养和植株再生。经实验筛选出最适合诱导愈伤组织的培养基是Ms＋6-BA1．0～2．0＋NAA0．1～0．5；脱分化的最佳激素组合是培养基Ms＋6-BA1．0＋NAA0．5。eaves of Kim Jong-il Begonia tuberhybrieda Vosa were used as explants to conduct tissue culture induction. Ms medium was basic medium,adding with different concentrations of cytokinin 6-BA and NAA or 2 ,4-D to carry on plant tissue culture and regeneration. The experiment selected the most suitable callus induction of the medium was MS＋BA1.0-2.0＋NAA0.1-0.5;dedifferentiation hormone combination was the best medium MS＋BA1.0＋NAA0.5.

关 键 词：球根海棠 组织培养 诱导 

分 类 号：S682.29[农业科学—观赏园艺]