洁泽2号预防滴虫性阴道炎的实验研究  

The experimental study of Jieze No 2 on trichomonas vaginitis

作　　者：陈琢[1] 王容[1] 孔小锋[1] 陆付耳[1] 马永贵[2] 黄光英[3] 

机构地区：[1]华中科技大学同济医学院附属同济医院中西医结合科,湖北武汉430030 [2]华中科技大学同济医学院附属同济医院药学部 [3]华中科技大学同济医学院中西医结合研究所

出　　处：《中国妇幼保健》2009年第2期241-244,共4页Maternal and Child Health Care of China

基　　金：国家十五科技攻关项目(2004BA709B13-02)

摘　　要：目的:观察洁泽2号(JZ2)体外抗阴道毛滴虫的药效及其对小鼠滴虫性阴道炎的预防作用。方法:利用不同浓度的JZ2、JZ1、N-9及甲硝唑进行体外抗滴虫实验,观察不同时间滴虫的死亡率;将小鼠随机分为JZ2组(高、中、低)、JZ1组(高、中、低)、壬苯醇醚(N-9)组、甲硝唑组、空白凝胶组、模型组。接种滴虫液,分别于给药后2、4、7、10、12、14天取小鼠阴道冲洗液涂片镜检及培养,观察感染情况,并计算不同时间各组的感染率。结果:随着药物作用时间的延长和药物浓度的增加,滴虫死亡率增高;同一时间下,药物各浓度组滴虫死亡率与对照组比较均有显著性差异(P<0.05);JZ2以(0.25±0.0625)mg·ml-1浓度作用2h及(0.125±0.03125)mg·ml-1浓度作用48h后滴虫死亡率均达到100%,相同时间内滴虫死亡率高于同浓度的JZ1、N-9及1%浓度的甲硝唑(P<0.05)。体内实验JZ2组、N-9组感染率为0,与相同时间模型组比较差异有显著性(P<0.01);甲硝唑组感染率前4天仅为10.0%,前10天感染率与模型组比较差异有显著性(P<0.01);JZ1组滴虫感染率比模型组低,2g·ml-1浓度组在第2天与模型组比较差异有显著性(P<0.05);空白凝胶组在不同时间的滴虫感染率与模型组比较,差异均无显著性(P>0.05);JZ2组、N-9组、JZ12g·ml-1和1g·ml-1剂量组滴虫感染率与甲硝唑组比较,差异均无显著性(P>0.05);在0.5g·ml-1浓度下,JZ2组与JZ1组比较第2天感染率,差异有显著性(P<0.05)。结论:JZ2有较好的体外杀滴虫作用,其效果优于同浓度的JZ1、N-9及1%的甲硝唑;预防性给药后,JZ2高、中、低剂量组和N-9组都能完全预防小鼠滴虫的感染,其作用强于同浓度的JZ1。Objective： To observe the effect of Jieze No. 2 Decoction on trichomonas vaginalis in vitro and preventing effects of Jieze No. 2 gel on trichomonas vaginalis in mice. Methods： Trichomonaeidal effect was investigated using various concentrations of Jieze No. 2 Decoction, N -9, Jieze No. 1 Decoction and Metronidazole. The mortality was observed under microscope. 112 mice were separated into Jieze No. 2 group （ high titer, median titer and lower titer）, Jieze No. 1 group （ high titer, median titer and lower titer）, Nonoxynol - 9 group, Metronidazole group, jel control group and model group. Every mouse was inoculated by 20 ul jel of different drugs or saline intravaginally before modeling composition, 2min later, all mice were inoculated by 20ul trichomonas vaginalis of trophozoites amount of 1 ×10^7 ml^-1. We collected the lotion to test under microscope and culture in the medium at 2, 4, 7, 10, 12, 14 day after inoculation, checking ac- tive trichomond and comparing infection rate between each group. Results： With prolongation of the time of drug action and increase of its concentration, the mortality of T. vaginalis increased. The death rates of T. vaginalis in altimeter with different concentration groups were higher than that of control groups at the same time P 〈 0. 05 ） ; After treated with Jieze No. 2 Decoction of （ 0. 25 ± 0. 062 5 ） mg·ml^-1 concentration for 2 h or （0. 125 ±0. 031 25 ） mg/ml^-1 concentration for 48 h, the mortality was reached 100%. Its mortality was higher than that of N -9 and Jieze No. 1 Decoction at the same concentration and metronidazole of 1/100 concentration （P 〈 0. 05 ） . The infection rate of Jieze No. 2 and N - 9 group was 0. 0%, it had significant difference compared with model group at the same time （ P 〈 0. 01 ） ; The infection rate of metronidazole group was 10% before 4 days, it was lower than that of control group before 10 days （P 〈0. 01 ） ; The infection rate of Jieze No. 1 2 g·ml^-1 group was lower than that of control

关 键 词：洁泽2号 洁泽1号 壬苯醇醚 滴虫性阴道炎 

分 类 号：R453[医药卫生—治疗学] R711.31[医药卫生—临床医学]