妊娠高血压疾病孕妇胎盘凋亡相关基因的表达  

Expression of apoptotic gene in placenta tissue of hypertensive disorder complicating pregnancy

作　　者：滕红[1] 徐晓红[1] 韩凝[1] 孙红霞[1] 

机构地区：[1]吉林大学第二医院妇产科,130041

出　　处：《中国妇幼保健》2009年第4期552-556,共5页Maternal and Child Health Care of China

基　　金：吉林省科技厅科研课题(200505121)

摘　　要：目的:探讨正常妊娠及妊娠高血压疾病(HDCP)孕妇胎盘组织凋亡相关基因Survivin、Bcl-2和Caspase-3的表达及其相互关系。方法:应用免疫组化的方法(S-P法)检测101例HDCP(其中轻度子痫前期51例、重度子痫前期50例,分别为轻度组、重度组)及62例正常晚孕(对照组)胎盘组织Survivin、Bcl-2和Caspase-3的表达。结果:①Survivin主要定位于绒毛细胞滋养细胞胞浆内,在重度组表达显著低于轻度组及对照组(P<0.05);②Bcl-2主要定位于合体滋养细胞胞浆内,Bcl-2阳性表达在各组之间无显著性差异(P>0.05)。③Caspase-3主要定位于合体滋养细胞及细胞滋养细胞胞浆内,Caspase-3表达在轻度组、重度组显著高于对照组(P<0.01)。④Survivin与Caspase-3在两组胎盘组织中表达呈负相关(r=-0.208,P=0.008<0.01);Bcl-2与Caspase-3在两组胎盘组织中表达呈负相关(r=-0.199,P=0.011<0.05);Sur-vivin与Bcl-2基因表达无相关性(P>0.05)。结论:在妊娠高血压疾病患者胎盘组织中Survivin基因表达下调、Caspase-3基因表达上调、Bcl-2基因表达无明显变化。妊娠高血压疾病的发病机制可能与Survivin及Caspase-3表达变化有关。Objective： To investigate the expression of Survivin, Bcl -2 and Caspase -3 in placenta tissue of hypertensive disor- der complicating pregnancy （HDCP） . Methods ： 101 placenta tissues of preeclampsia （ including 51 mild preeclampsia as low - grade group and 50 severe preeclampsia as high -grade group） and 62 placenta tissues of normal pregnancy （control group） were detected for the expression of Survivin, Caspase - 3 and Bcl - 2 by immunohistochemistry assay. Results ： ①Expreesion ratio of Survivin in high - grade group was significantly lower than thai in low - grade group and that in control group （ P 〈 0.05 ） .② There was no significant difference in the expression ratio between three groups （P〉0.05）.③ Expression ratios of Caspase - 3 in high - grade group and low - grade group were significantly higher than that in control group （P 〈0.01） , there was no significant difference between high -grade and low -Grade group （ P〉0.05）. ④Expression of Caspase - 3 was negatively related to expression of Survivin （ P 〈 0. 01 ）, expression of Bcl-2 was negatively related to expression of Caspase-3 （r=-0. 199, P〈0.05 ） , there was no relationship between the expression of Survivin and Bcl-2 （P〉0.05） . Conclusion ： Down-regulation of Survivin and up - regulation of Caspase-3 may play an important role in the pathogenesis of HDCP.

关 键 词：胎盘 细胞凋亡 SURVIVIN CASPASE-3 Bcl-2 

分 类 号：R714[医药卫生—妇产科学]