黄曲霉侵染前后花生过氧化物酶与过氧化氢酶活性变化的研究  

Activity Changes of Peroxidase and Catalase in Peanut Before and After Invaded by Aspergillus Flavus

作　　者：陈高[1,2] 闫彩霞[1] 李春娟[1] 张廷婷[1] 单世华[1] 

机构地区：[1]山东省花生研究所,山东青岛266100 [2]东北农业大学,黑龙江哈尔滨150030

出　　处：《山东农业科学》2009年第3期12-14,共3页Shandong Agricultural Sciences

基　　金：国家自然基金项目(30771361);山东省农业科学院青年基金项目(2006YQN018)

摘　　要：采用分光光度法对黄曲霉侵染前后两种花生籽仁中过氧化物酶(POD)和过氧化氢酶(CAT)活性进行测定。试验结果表明,接种8 h后两花生品种POD活性明显提高,并表现出明显的活性高峰,之后POD活性逐渐下降,24 h后POD活性变化幅度较小,两花生品种间的POD活性相差较大;接种后随时间的延长CAT活性降低,24 h后趋于稳定,两花生品种CAT活性相差较大。两种酶之间的变化无明显的同步性,两花生品种中两种酶的活性变化趋势也不尽相同。The changes of the peroxidase and catalase activities in peanut before and after invaded by AsperfiUus flavus were analyzed. The results indicated that the POD activity of the two peanut varieties were obviously raised in 8 hours of inoculation and showed apparent peaks, and then the POD activity gradually decreased and tended to be stable after 24 hours of inoculation. The POD activity was different between the two peanut varieties. There was a significant negative correlation between the CAT activity and the inoculating time, the CAT activity tended to be stable after 24 hours of inoculation and there was larger difference between the two peanut varieties. The changes of POD and CAT activities had no significant synchronization and also had different trend.

关 键 词：花生 黄曲霉侵染 过氧化物酶活性 过氧化氢酶活性 

分 类 号：S565.201[农业科学—作物学]