醛糖还原酶对211首糖尿病中药复方作用的筛选研究  

Screening of Actions of 211 Herbal Formulae for Diabetes with Aldose Reductase

作　　者：柯向梅[1] 张彦廷 张彦玲 吴中秋[1] 张再康[1] 牛彦平[4] 

机构地区：[1]河北医科大学中医学院中医系中医临床基础教研室,河北石家庄050091 [2]华北制药集团新药研究开发公司天然药物研究室,河北石家庄050015 [3]河北省赞皇县医院医务科,河北石家庄051230 [4]河北医科大学第二医院,河北石家庄050000

出　　处：《上海中医药杂志》2009年第3期76-78,共3页Shanghai Journal of Traditional Chinese Medicine

基　　金：河北省科技攻关计划课题(07276101D-7)

摘　　要：目的通过醛糖还原酶高通量模型对211首中药复方的筛选,寻找治疗糖尿病的科研配方。方法从猪晶状体中提取醛糖还原酶,通过测定340nm处光密度的下降速率,间接测定醛糖还原酶的活性的方法建立的高通量醛糖还原酶筛模型的筛选,比较211首方剂的6 630个制备样品对醛糖还原酶的抑制作用。结果其中4首方中5组制备样品对醛糖还原酶有特异性的抑制作用。结论中药复方应用醛糖还原酶高通量模型筛选评价,简便快捷,可以优选得到科研配方。Objective To select scientific herbal formulae for diabetes in high flux model by screening the actions of 211 herbal formulae with aldose reductase. Methods Extract aldose reductase from pig lens, determine indirectly the method of enzymatic active aldose reductase by measuring slowdown rates at optical density 340 nm and compare the repression of aldose reductase of 6630 prepared samples in 211 herbal formulae. Results Five groups of the prepared samples out of the 4 herbal formulae could specifically inhibit the aldose reductase. Conclusion Screening method of high flux model with aldose reductase is convenient and efficient in selecting herbal formulae for diabets.

关 键 词：糖尿病并发症 醛糖还原酶 高通量筛选 中药复方 

分 类 号：R285[医药卫生—中药学]