不同剂量辐射损伤后T淋巴细胞亚群,TH1和TH2变化观察  

Observation the Changs of T-Lymphocyte Subsets CD3,CD4,CD8,Th1 and Th2 After Exposure to Various Dose of Irradiation

作　　者：郑辉[1] 涂序珉[1] 甄荣[1] 张志兴[1] 

机构地区：[1]中国疾病预防控制中心辐射防护与核安全医学所,北京100088

出　　处：《中国辐射卫生》2009年第1期13-15,共3页Chinese Journal of Radiological Health

摘　　要：目的观察不同剂量辐射损伤对小鼠T淋巴细胞功能亚群的影响,探讨辐射所致的免疫系统损伤在细胞学和分子水平上的机制。方法C57BL/6j小鼠采用60Coγ射线照射诱导辐射损伤模型。总照射剂量分别为0.7、1.4、2.8、和5.6Gy。应用细胞表面标志和细胞内因子标记,流式细胞仪检测分析不同剂量组小鼠脾T淋巴细胞功能亚群CD3,CD4,CD8改变及Th1和Th2的变化。结果1.小鼠脾T-淋巴细胞功能亚群CD3,CD4和CD8在照射后均有明显降低,降低幅度与受照剂量明显相关。2.CD4/CD8比值显示照射后均有明显升高。3.照射后Th1和Th2均有明显降低,其中Th1降低明显高于Th2。受照剂量大于2.8Gy组Th2/Th1比值较空白对照组明显升高。结论不同照射剂量对T淋巴细胞功能亚群CD3,CD4,CD8和CD4/CD8有明显影响,Th1和Th2和Th2/Th1功能亚群的失衡在辐射所致的免疫损伤中具有较重要作用。Objective To observe the effects of T - lymphocyte subsets CD3, CD4, CD8 and CD4/CD8 after different dose of irradiation and investigate the cellular and molecular mechanism of immune system injury induced by irradiation. Methotis The C57BL/6j mice were divided into normal control and model groups. The mice in radiation hurts model were induced by ^60Co gamma rays （0.7, 1.4, 2.8 and 5.6Gy）. The surface marker and intracellular cytokines of lymphocytes were stained with fluorescence labeled monoclonal antibodies . The changes of T - lymphocyte subsets CD3, CD4, CD8, IFN - r and interleukin - 4 in spleen celles were analyzed by flow cytometry. Results 1. The T - lymphocyte subsets CD3, CD4, CD8 have distinctly reduced after irradiation. This change range is related with gamma rays dose. 2. The CD4/CD8 is significantly increased. 3. The Thl and Th2 have depression after irradiation and the depression level of Thl is higher than Th2. When irradiation dose is over 2.8Gy, Th2/Thl shows marked increase compared with the control group. Conclusion It is suggested that the changes of mice T - lymphocyte subsets CD3, CD4, CD8 and CD4/CD8 ratio are caused by different dose after irradiation, especially the imbalance of Thl ,Th2 and Th2/Thl ratio plays an important role in radiation immune harm.

关 键 词：辐射免疫损伤 T淋巴细胞亚群 淋巴细胞因子 Th1 Th2 

分 类 号：R816.3[医药卫生—放射医学]