苎麻纤维素合成酶基因cDNA的功能分析  

Function analysis of cellulose synthase gene cDNA of ramie

作　　者：易蓉[1] 田志坚[1] 黄丽华[1] 刘峰[2] 郭清泉[2] 张学文[1] 

机构地区：[1]湖南农业大学生物科学技术学院,湖南长沙410128 [2]湖南农业大学苎麻研究所,湖南长沙410128

出　　处：《湖南农业大学学报（自然科学版）》2009年第1期13-16,共4页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：国家自然科学基金项目(30571186);湖南农业大学人才科学基金项目(05WD16)

摘　　要：运用克隆的苎麻纤维素合成酶(BnCesA1)基因cDNA及植物表达载体pWM101,构建了35S启动子控制的苎麻BnCesA1基因核心区段反义融合表达载体(pWM-BnCesA),并通过根癌农杆菌介导法将其转化至模式烟草WS38,获得了转基因烟草.对转基因烟草植株进行的分子检测和初步组织学研究表明,转反义BnCesA1基因核心区段对纤维素的合成产生干扰,植株叶柄切片初生组织细胞较野生烟草松散,其基本组织细胞涨大,间隙也相应增大,证实BnCesA1基因的纤维素合成功能,表明BnCesA1基因在植物初生组织和次生组织的细胞壁合成中都发挥作用.Cellulose synthase is the key enzyme in ramie cellulose biosynthesis. We cloned the putative cDNA of cellulose synthase of ramie （BnCesAl）. In this study the core region of the cDNA was inserted into a Ti plasmid vector pWM101 in anti-sense direction under the control of the 35S promoter. The recombinant was transformed into Nicotiana tabacum WS38 via Agrobacterium tumefaciens mediated transformation. Transgenic tobacco seedlings are screened out. Histology investigation of the transgenic showed that the anti-sense BnCesAl gene expression in tobacco could interfere its cellulose biosynthesis. The cells in cross-section of leaf stalk of transgenic appear swollen shape. The increased intercellular space in ground tissue of the transgenic was also observed. It indicated that BnCesAl gene plays a role in the process of cellulose biosynthesis in plant and it is functional both in primary and secondary cellulose synthesis.

关 键 词：苎麻 纤维素合成酶基因cDNA 反义RNA转化 功能分析 

分 类 号：Q786[生物学—分子生物学]