细胞滋养细胞中DPPⅣ的表达及其与侵袭能力的关系  

Study on relationship between DPPⅣ and invasive ability of trophoblast cell

作　　者：李幼飞[1] 李力[1] 俞丽丽[1] 郑英如[1] 

机构地区：[1]第三军医大学大坪医院野战外科研究所妇产科,重庆400042

出　　处：《重庆医学》2009年第6期638-640,共3页Chongqing medicine

摘　　要：目的了解妊娠过程中细胞滋养细胞表面表达的二肽基肽酶(DPPⅣ)与细胞侵袭能力的关联。方法采用流式细胞术检测培养的早孕绒毛细胞滋养细胞和绒癌细胞株JAR中DPPⅣ蛋白的表达;并利用Transwell侵袭分析系统观察两种细胞侵袭能力的差异。结果原代培养细胞和JAR细胞上均表达DPPⅣ蛋白,但原代培养细胞上的表达量高于JAR细胞,相反,JAR细胞的侵袭能力高于原代培养细胞。结论证明了DPPⅣ与滋养细胞的侵袭性特性有关,DPPⅣ的表达量与细胞的侵袭能力呈负相关,从而为子痫前期致病机理研究提供一定的理论依据。Objective To understand the relationship between DPPⅣ expressed on the surface of trophoblast ceil and the invasive ability of the cell. Methods The flow cytometry was used to inspect the DPPⅣ＇s protein expression from the cultivated trophoblast cells of the early gestation villi and the JAR ceil. The Transwell invasion system was also used to to observe the difference on invasion ability between the two cells. Results Both the primary cultivated cell and JAR cell expressed DPPⅣ protein,but the primary cultivated cell expressed more than JAR cell. On the contrary, the invasive ability of JAR cell was stronger than that of the primary cultivated cell. Conclusion DPPⅣ is related to the invasive phenotype of the trophoblast cell,and the expression of DPPⅣ is negatively correlated with its invasive ability. Thus it provides some theoretical basis for the study of preeclampsia pathogenic mechanism.

关 键 词：二肽基肽酶 细胞滋养细胞 侵袭 

分 类 号：R714[医药卫生—妇产科学]