31份野生树莓资源的RAPD分析  

Analysis on 31 wild raspberries with RAPD markers

作　　者：李媛媛[1] 郭修武[1] 代汉萍[1] 刘海涛[1] 

机构地区：[1]沈阳农业大学园艺学院,沈阳110161

出　　处：《果树学报》2009年第2期240-244,共5页Journal of Fruit Science

基　　金：农业部科技部行业科研专项(nykyzx07-028);农业部"十一五"948项目G-25

摘　　要：用RAPD技术分析了31份野生树莓资源的亲缘关系,从100个A系列随机引物中选出了15个多态性高、分辨能力强的引物。将这15个引物应用于31份野生树莓材料,共扩增出182条谱带,其中170条为多态性标记(占93.41%),平均每个引物扩增多态性谱带12.13条,表明不同树莓资源基因型间存在着极为丰富的遗传多样性。基于RAPD的扩增结果建立UPGMA聚类分析树状图,31份野生树莓资源在遗传距离0.5100处被划分为4个类群。从分子水平研究了树莓属资源的亲缘关系,为树莓种质资源的保存和利用及其杂交亲本的选配提供了依据。A total of 31 raspberry resources were analyzed by random amplified polymorphic DNA markers. 15 primers were screened from 100 random decamer primers, and a total of 182 DNA bands were amplified, 170 of which （93.41%） were polymorphic. The average number of polymorphic DNA bands amplified by each primer was 12.13, which showed abundant genetic diversity among different raspberry genotypes. According to the data, a dendrogram of genetic relationship was constructed using UPGMA method in which all the tested resources were classified into four cluster groups with the Nei＇s genetic distance of 0.51. This study revealed the genetic relationship of wild raspberry resources at molecular levels, which provids useful information for germplasm storage, utilization, and parents selection for breeding.

关 键 词：树莓 RAPD 亲缘关系 聚类分析 

分 类 号：S663.2[农业科学—果树学]