BMP-2诱导肌源性干细胞转化为软骨样细胞的体外实验研究  

Study of Chondrocytic Differentiation of Muscle-derived Stem Cells in Vitro

作　　者：梅晰凡[1] 袁亚江[1] 吕刚[1] 郭占鹏[1] 李全双[1] 

机构地区：[1]辽宁医学院附属第一医院骨科,辽宁锦州121000

出　　处：《中国医科大学学报》2009年第2期92-95,共4页Journal of China Medical University

基　　金：辽宁省博士启动基金资助项目(20081100)

摘　　要：目的体外定向诱导大鼠肌源性干细胞(MDSCs)向软骨细胞转化,并对诱导细胞进行鉴定。方法用差速贴壁的方法对SD大鼠MDSCs进行分离和培养。用骨形态发生蛋白2(BMP2)将MDSCs进行诱导分化,9d后取出标本,行甲苯胺蓝染色检测软骨基质的分泌,免疫组织化学检测软骨特异性Ⅱ型胶原表达。RT-PCR检测Ⅱ型胶原和聚集蛋白聚糖(aggrecan)的mRNA表达。结果5d后镜下见细胞形态由梭形向多边多角形转化。9d后诱导组甲苯胺蓝染色阳性,Ⅱ型胶原免疫组化检测阳性,RT-PCR检测Ⅱ型胶原和aggrecan mRNA呈阳性表达。结论BMP-2可诱导MDSC向软骨细胞转化。Objective To induce muscle-derived stem cells （MDSCs） of rats to differentiate directionally towards chondrocytes in vitro and identify the differentiated cells. Methods MDSCs were isolated from SD mrs and cultured in vitro with differential attachment method.BMP- 2 was used to induce transformation of MDSCs from the third passage. After 9 days of induction, specific markers of differentiation of chondrocytes were detected by the appearance of toluidine blue staining and immunohistochemistry detection of collagen type Ⅱ. Results The induced MDSCs changed into triangle or polygonal shape from spindle shape after 7 days.Specific markers of chondrocytes were positive in the appearance of toluidine blue staining,immunohistochemistry detection of collagen type Ⅱ , and RT-PCR detection of collagen type Ⅱ and aggrecan mRNA. Conclusion BMP-2 can induce MDSCs to differentiate into chondrocytes in vitro.

关 键 词：肌源性干细胞 软骨细胞 细胞分化 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]