TGF-β_1对胃癌细胞BGC-823侵袭性的影响  

The influence of TGF-β_1 on invasive ability of BGC-823

作　　者：刘栗丽[1] 吴继锋[1] 祝迎锋[1] 张红[1] 秦蓉[1] 赵文娣[1] 

机构地区：[1]安徽医科大学病理学教研室,合肥230032

出　　处：《安徽医科大学学报》2009年第1期18-22,共5页Acta Universitatis Medicinalis Anhui

基　　金：安徽省教育厅重点科研项目基金(编号:kj2007A029)

摘　　要：目的研究转化生长因子β1(TGF-β1)对人胃癌细胞BGC-823侵袭性的影响。方法将体外培养的人胃癌细胞BGC-823用外源性人重组TGF-β1干预后,用四甲基偶氮唑蓝比色法(MTT法)检测TGF-β1对细胞增殖的影响;免疫细胞化学法、免疫荧光法、流式细胞术分别检测Snail、E-cadher-in及N-cadherin蛋白的表达;Transwell侵袭实验检测TGF-β1对细胞侵袭性的影响。结果①经过18h的作用,低浓度(1、5和10ng/ml)的TGF-β1促进胃癌细胞的增殖,高浓度(20和50ng/ml)的TGF-β1则抑制胃癌细胞的增殖,且呈剂量依赖性;选取不同时间段(18、42、66h)检测细胞生长曲线,发现不呈时间依赖性;②在外源性TGF-β1作用下,胃癌细胞均不同程度地表达Snail、E-cadherin及N-cadherin,其中TGF-β1对Snail、N-cadherin表达有促进作用,而对E-cadher-in的表达有抑制作用,E-cadherin和N-cadherin的表达呈相反的趋势;③选取不同浓度的TGF-β1(1、10和20ng/ml),发现10ng/ml的TGF-β1明显促进胃癌细胞的侵袭性(P<0.05)。结论不同浓度的TGF-β1对胃癌细胞增殖的影响不同;TGF-β1有增加胃癌细胞侵袭性的作用,该作用可能是通过上皮间质转化而产生,钙黏蛋白表达的变化可能是重要的分子学机制之一。Objective To investigate the influence of TGF-β1on invasive ability of the gastric adenocarcinoma cell line BGC-823 in vitro. Methods Human low-differentiated gastric adenocarcinoma cell line BGC-823 was cultured in vitro. After exposed to different concentration of TGF-β1, the proliferation of BGC-823 cells was measured by MTT assay. The expression of Snail, E-cadherin and N-eadherin was detected by immunocytochemical staining （ ICC）, immunofluorescence assay （IFA） and flow cytometry （FCM）. The changes of invasion ability of BGC-823 were detected by transwell cell culture chambers. Results I. After exposured to TGF-β1 （ 1, 5 and 10 ng/ml） for 18 h, the growth of BGC-823 cells was promoted dose-dependently. Meanwhile the inhibitory effect of TGF-β1 （20 and 50 ng/ml） was observed unexpectedly. Then the cell growth curve was measured at different time （18, 42, 66 h）. As a result, it didn＇t show time-dependent manner. 2. The expression of Snail, E-eadherin and N-cadherin was measured at different levels after culture in the presence of human recombinant TGF-β1. The opposite trend was showed between E-cadherin and N-eadherin. TGF-β1 inhibited the expression of E-eadherin, but enhanced that of N-cadherin and Snail. 3. The changes of invasion were detected in BGC-823 cells exposed to TGF-β1 （ 1, 10 and 20 ng/ml）, the rate of the changes was significantly high at dose of 10 ng/ml. Conclusion The proliferation of BGC-823 cells is determined by different concentrations of TGF-β1. The TGF-β1 does have the ability to activate EMT in gastric adenocarcinoma cells, thus increase their invasion. The eadherins switch is one of the important molecular mechanisms in this phenomenon.

关 键 词：胃肿瘤/病理学 转化生长因子Β 肿瘤细胞 培养 

分 类 号：R392.11[医药卫生—免疫学] R329.2[医药卫生—基础医学]