中草药中多糖的测定方法改进  

Determination of the Content of the Deoxidize Sugar in Leptopus chinese(Bunge) Pojark

作　　者：陈彦君[1,2] 王武鹏[1] 潘顺浩[1] 龙跃[1] 王桂红[1] 

机构地区：[1]郑州大学,河南郑州450052 [2]焦作大学,河南焦作454003

出　　处：《时珍国医国药》2009年第3期608-609,共2页Lishizhen Medicine and Materia Medica Research

基　　金：河南省科委攻关基金(No.02246300110624420031)

摘　　要：目的测定雀儿舌头中多糖的含量。方法根据蒽酮硫酸试剂可与游离的寡糖、多糖中的己糖、戊糖及其糖醛和糠醛酸起显色反应，采用紫外分光光度法测定雀儿舌头中多糖的含量。结果该法的线性范围为40～320μg，r=0．9977，平均回收率为101．2％，RSD为1．35％。结论该法简便、快速、准确、重复性好，可作为雀儿舌头中多糖含量的测定方法。Objective To determine the content of the deoxidize sugar in Leptopus chinese （Bunge） Pojark. Methods The content of the deoxidize sugar in Leptopus chinese （Bunge） Pojark was determined using sulfuric aeidearbolic acid method. Results The linear range was 40 - 320 p.g （ r = 0. 997 7 ）. The average recovery rate was 101.2.5%. RSD was 1.35%. Conclusion This method is simple, quick, accurate and reproducible, thus it can be used for determining the content of the deoxidize sugar in Leptopus chinese （Bunge） Pojark.

关 键 词：雀儿舌头 紫外分光光度 多糖 

分 类 号：O637.32[理学—高分子化学]