随机扩增多态性DNA标记技术及其在药用植物研究中的应用  

RAPD Technology and its Application in Medicinal Plant Research

作　　者：王晓慧[1,2] 汤晓闯[1,2] 杨恩秀[1] 姜程曦[1,2] 董建勇[2] 黄志锋[2,3] 

机构地区：[1]吉林农业大学生物反应器与药物开发教育部工程研究中心,吉林长春130118 [2]温州医学院药学院,浙江温州325035 [3]瑞安市温医沙洲温莪术服务有限公司,浙江瑞安320000

出　　处：《时珍国医国药》2009年第3期618-620,共3页Lishizhen Medicine and Materia Medica Research

基　　金：浙江省科技攻关重大资助项目(No.2005C13019);浙江省瑞安市科技计划项目(No.200673-76);浙江省中医药局项目(No.2008CB049)

摘　　要：随机扩增多态性DNA(RAPD)技术是近年来广泛应用的分子标记技术之一,以PCR反应为基础,其特点是快速简便、易操作、成本较低,DNA需要量少、无需放射性分析,也不会污染等。该文简述了RAPD技术的原理及优缺点以及其在药用植物遗传多样性分析、药材鉴别和DNA指纹图谱的构建、亲缘关系及系统学研究、药材的道地性等方面的应用,并展望了其发展前景。RAPD technology was widely used in molecular marker, based on PCR reaction. Their characteristics was simple, rapid , easy to operate, with low cost. It need less DNA and no radioactive analysis, and it has no pollution. This paper introduces the principle of RAPD technology, advantages and disadvantages as well as their genetic diversity in medicinal plants, herbal medicine identification and DNA fingerprinting construction, relationship and systems research, the genuineness of medicine, and prospect of RAPD.

关 键 词：随机扩增多态性DNA 遗传多样性 药材鉴别 道地性 

分 类 号：R2-03[医药卫生—中医学] R282.5