羟基积雪草苷对离体动脉条的舒张作用及其机制  

Effects and Mechanisms of Madecassoside on Isolated Thoracic Aortic Rings of Rats

作　　者：白纪红[1] 赵日红[1] 吕秋军[2] 马宪梅[2] 

机构地区：[1]宜春学院·医学院,江西宜春336000 [2]中国人民解放军军事医学科学院放射与辐射医学研究所,北京100850

出　　处：《时珍国医国药》2009年第3期674-676,共3页Lishizhen Medicine and Materia Medica Research

摘　　要：目的探讨羟基积雪草苷(madecassoside,MC)的舒张血管作用及其机制。方法采用离体大鼠胸主动脉张力实验观察MC的舒张血管作用。结果MC使去甲肾上腺素(NE)和氯化钾(KC l)收缩动脉条的量效曲线右移,最大收缩力降低;并对NE诱导的细胞内Ca2+释放所引起的收缩有明显的抑制作用;但去除血管内皮细胞后MC反而使动脉条收缩进一步增强;在有一氧化氮合酶(NOS)抑制剂存在的情况下,其抑制NE收缩的效应消失;对血管紧张素Ⅱ(AngⅡ)收缩动脉条的量效曲线无明显影响。结论MC有明显的舒张血管作用,表现为钙拮抗活性,以抑制细胞内Ca2+释放为主;且通过内皮细胞发挥作用,并与一氧化氮(NO)合成通路有密切的关系。Objective To investigate the effects and mechanisms of the vasorelaxation of madecassoside （MC）. Methods The vasodilating action of MC was studied using isolated rat thoracic aortic rings. Results MC caused rightward shift of the concentration - response curves for NE and KCl and the decrease of maximal response, significantly reduced the Ca^2＋ release from intracellular calcium storage induced by NE ; Pre - treatment with Nitric oxide synthase inhibitor significantly inhibited the relaxation induced by MC. There was no significant influence on the concentration - response curves for Angiotensin Ⅱ. Conclusion The above results indicated that MC could relax vascular smooth muscle in a endothelium - dependent manner. The mechanism may be related with its calcium - antagonistic effect, inhibition of the Ca^2＋ release from intracellular calcium storage , and related to nitric oxide system.

关 键 词：羟基积雪草苷 去甲肾上腺素 一氧化氮合酶抑制剂 内皮细胞 胸主动脉 

分 类 号：R285.5[医药卫生—中药学]