饮食诱导肥胖大鼠蛋白酪氨酸磷酸酶1B表达的变化  

Changes of expression of protein tyrosine phosphatase 1B in rats with diet-induced obesity

作　　者：卢斌[1] 郭志福[1] 邹大进[1] 

机构地区：[1]第二军医大学长海医院内分泌病科,上海200433

出　　处：《江苏医药》2009年第3期296-298,I0005,共4页Jiangsu Medical Journal

基　　金：国家自然科学基金资助项目(30700380)

摘　　要：目的观察饮食诱导肥胖大鼠骨骼肌、肝脏、胰腺蛋白酪氨酸磷酸酶1B表达变化,探讨蛋白酪氨酸磷酸酶1B在肥胖胰岛素抵抗发生中的作用。方法雄性SD大鼠20只,随机均分为正常饲养(NC)组和高脂饲养(HF)组,喂养8周,比较两组大鼠体重、胰岛素抵抗指数(HOMA-IR)及骨骼肌、肝脏、胰腺蛋白酪氨酸磷酸酶1B的表达。结果HF组大鼠体重比NC组显著增加,并出现胰岛素抵抗(HOMA-IR8.58±3.37vs.3.46±2.21)(P<0.01)。HF组大鼠骨骼肌、肝脏、胰腺蛋白酪氨酸磷酸酶1B表达量较NC组显著增加(P<0.01)。结论高脂饮食可诱导大鼠肥胖伴胰岛素抵抗,其胰岛素抵抗的形成可能与蛋白酪氨酸磷酸酶1B表达水平的升高有关。Objective To investigate the changes of expression of protein tyrosine phosphatasc 1B（PTP1B） in rats with diet-induced obesity, and understand the role of PTP1B in the development of insulin resistance. Methods Twenty male SD rats were divided into two groups of taking normal chow （group NC） and high-fat diet （group HF） with 10 rats each. Eight weeks later, body weight, fasting glucose, fasting insulin and lipid profile were determined and HOMA-IR was calculated. PTP1B levels in muscle, liver and pancreas were measured by immunohistochemical method. Results Rats of group HF showed significantly the body weight gain （P〈 0.01） and insulin resistance （HOMA-IR was 8.58±3.37 for group HF and 3.46±2.21 for group NC, P〈0. 01）. Compared with group NC, the levels of PTP1B in the muscle, liver and pancreas were significantly higher in group HF （P〈0.01）. Conclusion Obese rats with insulin resistance have been induced successfully with high-fat diet and the state of insulin resistance of obese rats is correlated with the increased levels of PTP1B in the muscle, liver and pancreas.

关 键 词：胰岛素抵抗 肥胖 蛋白酪氨酸磷酸酶1B 

分 类 号：R589[医药卫生—内分泌]